Spread of extended-spectrum beta-lactamase producing Escherichia coli isolates in Swedish broilers mediated by an incl plasmid carrying blaCTX-M-1
Spread of extended-spectrum beta-lactamase producing Escherichia coli isolates in Swedish broilers mediated by an incl plasmid carrying blaCTX-M-1
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DOI：
10.1186/1751-0147-55-3
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发表时间：
2013-01-21
影响因子：
2.1
通讯作者：
Englund, Stina
中科院分区：
文献类型：
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作者：
Borjesson, Stefan;Bengtsson, Bjorn;Englund, Stina
背景资料：欧洲肉鸡群体中产超广谱β-内酰胺酶（ESBL）大肠埃希菌的发生率已经很高且不断增加，这是令人担忧的，因为第三代和第四代头孢菌素被认为在人类医学中至关重要。瑞典34%的肉鸡携带产ESBL/pAmpC大肠杆菌。大肠杆菌，尽管缺乏已知的选择压力，如抗菌剂的使用。本研究的目的是筛选出一种E.大肠杆菌菌株携带的bla（CTX-M-1），以确定是否传播是由于一个特定的cloning.Findings：10株携带bla（CTX-M-1）从瑞典肉鸡属于8个不同的多位点序列类型与3株属于ST 155。通过PFGE评估，ST 155分离株相同。所有菌株的bla（CTX-M-1）均携带在一个复制子类型的质粒上，该质粒也可传递对四环素和磺胺甲恶唑的耐药性。大肠杆菌在瑞典肉鸡中的感染不是由于单个克隆的出现，而是由于携带bla的特异性incI质粒（CTX-M-1）的传播。
Background: The already high and increasing occurrence of extended-spectrum beta-lactamases (ESBL) producing Escherichia coli in European broiler populations is of concern due to the fact that third and fourth generation cephalosporins are deemed critically important in human medicine. In Sweden 34% of the broilers carry ESBL/pAmpC producing E. coli in their gut, despite the absence of a known selection pressure such as antimicrobial usages. The aim of the current study was to characterise a selection of E. coli strains carrying the bla(CTX-M-1), to determine if the spread was due to a specific clone.Findings: Ten isolates carrying bla(CTX-M-1) from Swedish broilers belonged to eight different multi-locus sequence types with three isolates belonging to ST155. The ST155 isolates were identical as assessed by PFGE. The bla(CTX-M-1) was in all isolates carried on a plasmid of replicon type incl, which also transferred resistance to tetracycline and sulfamethoxazole.Conclusion: The occurrence of ESBL-producing E. coli in the Swedish broilers is not due to the emergence of a single clone, but rather the spread of a specific incI plasmid carrying bla(CTX-M-1).