Further characterization of the polynucleotide phosphorylase of Micrococcus luteus.
Further characterization of the polynucleotide phosphorylase of Micrococcus luteus.
复制标题
藤黄微球菌多核苷酸磷酸化酶的进一步表征。
DOI：
10.1093/nar/2.2.149
复制
发表时间：
1975
影响因子：
14.9
通讯作者：
M. Singer
中科院分区：
文献类型：
--
作者：
C. Letendre;M. Singer
The purification of polynucleotide phosphorylase from Micrococcus luteus by chromatography on phosphocellulose colums is described. This procedure offers several advantages over previous procedures. Previously determined molecular weights for Form-I enzyme and Form-T enzyme derived from Form-I by limited tryptic hydrolysis were confirmed as 2.7 and 2.3 times 10-5, respectively. Form-I appears homogeneous in the ultracentrifuge, but multiple active protein species are separable by polyacrylamide gel electrophoresis. The multiple species are probably the result of proteolysis. On polyacrylamide gel electrophoresis under denaturing conditions, Form-T yielded a single size of subunit of 71,000 daltons, and Form-I yielded several bands of different molecular sizes. These results differ from earlier determinations. The amino acid compositions of Form-I and Form-T are reported. Form-I contains only between 8 and 10 cysteine residues per molecule and Form-T half that many.
