Sample preparation with fiber‐in‐tube solid‐phase microextraction for capillary electrophoretic separation of tricyclic antidepressant drugs in human urine
Sample preparation with fiber‐in‐tube solid‐phase microextraction for capillary electrophoretic separation of tricyclic antidepressant drugs in human urine
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DOI：
10.1002/1522-2683(200109)22:17
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发表时间：
2001-09
期刊：
影响因子：
2.9
通讯作者：
K. Jinno;M. Kawazoe;Yoshihiro Saito;T. Takeichi;M. Hayashida
中科院分区：
文献类型：
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作者：
K. Jinno;M. Kawazoe;Yoshihiro Saito;T. Takeichi;M. Hayashida
固相微萃取（SPME）是一种无溶剂的样品制备技术，使用附着在熔融石英纤维表面的薄涂层作为萃取介质，通过与气相色谱（GC）耦合，已成功应用于各种化合物的分析。近年来，使用GC毛细管柱作为萃取介质的管内SPME也得到了发展，并与液相色谱（LC）结合用于预浓缩非挥发性化合物。本研究建立了毛细管电泳-固相微萃取联用系统，并对4种三环类抗抑郁药阿米替林、丙咪嗪、去甲替林和地昔帕明进行了富集分离。在优化条件下，即使萃取介质长度大大缩短，萃取效果也优于传统的管内固相微萃取。结果清楚地表明，纤维作为提取介质有效。对于这四种TCA的分离，使用β-环糊精作为缓冲添加剂的毛细管电泳分离已经被采用，并且还证明了所开发的系统在生物基质中的复杂样品混合物的分析中的应用。
Solid‐phase microextraction (SPME) is a solvent‐free sample preparation technique using a thin coating attached to the surface of a fused silica‐fiber as the extraction medium, which has been successfully applied to the analysis of a wide variety of compounds by coupling to gas chromatography (GC). In recent years, in‐tube SPME using GC capillary column as the extraction medium has also been developed and coupled with liquid chromatography (LC) for the preconcentration of nonvolatile compounds. In this study, an on‐line interface between the fiber‐in‐tube SPME and capillary electrophoresis (CE) has been developed, and the preconcentration and separation of four tricyclic antidepressant (TCA) drugs, amitriptyline, imipramine, nortriptyline, and desipramine, were performed with the hyphenated system. Under the optimized condition, a better extraction performance than conventional in‐tube SPME was obtained, even the length of the extraction medium was much shorter. The results clearly indicated that the fiber was working effectively as an extraction medium. For the separation of these four TCAs, capillary electrophoretic separation with β‐cyclodextrin as the buffer additive has been employed and the application of the developed system to the analysis of complex sample mixtures in a biological matrix is also demonstrated.