Physicochemical properties and antioxidant activities of acidic polysaccharides from wampee seeds

Physicochemical properties and antioxidant activities of acidic polysaccharides from wampee seeds
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黄皮种子酸性多糖的理化性质及抗氧化活性

DOI:
10.1016/j.ijbiomac.2013.04.020
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发表时间:
2013-08-01
影响因子:
8.2
通讯作者:
Yang, Xihong
Yang, Xihong
中科院分区:
化学1区
文献类型:
--
作者:
Wu, Hui;Min, Tian;Yang, Xihong

文献摘要

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从黄皮[Clausena lansium(Lour.)Skeels]种子。研究了它们的理化性质、体内外抗氧化活性。结果表明,WP 1和WP 3均为酸性杂多糖,糖醛酸含量分别为45.35%和56.79%。WP 1的鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖的摩尔比为5.01:1.50:39.53:1:10.76:23.50,WP 3的鼠李糖、葡萄糖醛酸、半乳糖醛酸、葡萄糖、半乳糖、阿拉伯糖的摩尔比为5.76:1.73:27.27:1:4.72:8.77。WP 3不仅具有较WP 1更强的体外抗氧化活性,而且能显著提高高脂饮食诱导的SD大鼠血清中超氧化物歧化酶(SOD)活性和谷胱甘肽过氧化物酶(GSH-Px)水平。(C)2013爱思唯尔有限公司版权所有。
Two novel polysaccharide fractions, named WP1 and WP3, were extracted and purified from wampee [Clausena lansium (Lour.) Skeels] seeds. Their physicochemical properties, in vitro and in vivo antioxidant activities were investigated. Results indicated that both WP1 and WP3 were acidic heteropolysaccharides with uronic acid content of 45.35% and 56.79%, respectively. The molar ratio of rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, arabinose of WP1 was 5.01:1.50:39.53:1:10.76:23.50 while WP3 was 5.76:1.73:27.27:1:4.72:8.77. Furthermore, WP3 not only exhibited stronger in vitro antioxidant activities than WP1, but also more significantly increased the activities of superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) level in serum of SD rats induced by high-fat diet. (C) 2013 Elsevier B.V. All rights reserved.