A subset of beta-endorphin- or dynorphin-containing neurons in the medial basal hypothalamus accumulates estradiol.
A subset of beta-endorphin- or dynorphin-containing neurons in the medial basal hypothalamus accumulates estradiol.
复制标题
内侧基底下丘脑中含有β-内啡肽或强啡肽的神经元的子集会积累雌二醇。
DOI：
10.1159/000124212
复制
发表时间：
1985
影响因子：
4.1
通讯作者：
Pfaff,DW
中科院分区：
文献类型：
--
作者：
Morrell,JI;McGinty,JF;Pfaff,DW
我们使用类固醇放射自显影-免疫细胞化学相结合的方法来确定雌激素或地塞米松浓缩细胞是否含有内源性阿片肽。去卵巢-去肾上腺的雌性大鼠给予高放射性剂量的~3H-雌二醇或~H-地塞米松，然后处死以显示核类固醇结合。准备放射自显影，曝光2-12个月，照相显影，固定；用β-内啡肽或强啡肽A(1-17)的抗体在相同的切片上进行免疫细胞化学。在下丘脑内侧基底核，发现许多雌二醇和一些地塞米松集中神经元与β-Endor-Phin或强啡肽免疫反应阳性神经元混合。在下丘脑基底核内侧的β-内啡肽免疫反应神经元中，有4%的胞核中有雌二醇的浓集。此外，垂体前叶的β-内啡肽免疫反应细胞亚群在它们的核中浓缩了雌二醇。虽然下丘脑内侧基底核的β-内啡肽免疫反应神经元中没有地塞米松的浓集，但垂体前叶的β-内啡肽免疫反应细胞中有许多地塞米松的浓集。在下丘脑内侧基底核的强啡肽免疫反应神经元中，有10%的细胞胞核内有雌二醇。这些数据与雌激素对产生内源性阿片肽的下丘脑内侧基底神经元的特定子集产生基因组效应的假设是一致的。
We used the combined steroid autoradiography-immunocytochemical method to determine whether estradiol- or dexamethasone-concentrating cells contain endogenous opioid peptides. Ovariectomized-adrenalectomized female rats were given highly radioactive doses of3H-estradiol or3H-dexamethasone, then sacrificed to demonstrate nuclear steroid binding. Autoradiograms were prepared, exposed for 2–12 months, photodeveloped, and fixed; immunocytochemistry was carried out on the same sections using antibodies to β-endorphin or dynorphin A (1–17). In the medial basal hypothalamus, many estradiol- and some dexamethasone-concentrating neurons were found intermingled with β-endor-phin or dynorphin-immunoreactive neurons. Of the β-endorphin-immunoreactive neurons in the medial basal hypothalamus, 4% concentrated estradiol in their nuclei. In addition, a subset of β-endorphin-immunoreactive cells in the anterior pituitary concentrated estradiol in their nuclei. Although none of the β-endorphin-immunoreactive neurons in the medial basal hypothalamus concentrated dexamethasone in their nuclei, many of the β-endorphin-immunoreactive cells in the anterior pituitary did. Of the dynorphin-immunoreactive neurons in the medial basal hypothalamus, 10% concentrated estradiol in their nuclei. These data are consistent with the hypothesis of a genomic effect of estradiol on a particular subset of medial basal hypothalamic neurons that produce endogenous opioid peptides.