Crypt cells are involved in kin recognition in larval zebrafish
Crypt cells are involved in kin recognition in larval zebrafish
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DOI：
10.1038/srep24590
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发表时间：
2016-04-18
影响因子：
4.6
通讯作者：
Wullimann, Mario F.
中科院分区：
文献类型：
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作者：
Biechl, Daniela;Tietje, Kristin;Wullimann, Mario F.
斑马鱼幼虫的印记视觉和嗅觉亲属线索，分别在受精后第5和第6天，导致亲属识别以后的生活。暴露于非亲属线索会阻止印记和亲属识别。印记依赖于MHC II类相关信号，只有共享MHC II类等位基因的幼虫才能彼此印记。在这里，我们分析了哪种类型的嗅觉感觉神经元（OSN）检测亲属气味。单个硬骨鱼嗅上皮含有携带OR和TAAR基因家族受体的纤毛OSN（哺乳动物：主要嗅上皮）和具有V1R和V2R基因家族受体的微绒毛OSN（哺乳动物：犁鼻器）。此外，硬骨鱼显示具有微绒毛和纤毛的隐窝细胞。我们使用活性标记pERK（磷酸化细胞外信号调节激酶）刺激9日龄的斑马鱼幼虫后，无论是非亲属同种或食物气味。食物气味激活纤毛和微绒毛OSNs，只有后者被同种气味激活，而隐窝细胞对这两种刺激都没有激活。然后，我们测试了印迹和非印迹幼虫（全同胞）的亲属气味检测。我们提供了第一个直接的证据表明，隐窝细胞，可能是一个亚群的微绒毛OSNs，但不是纤毛OSNs，在检测一个亲气味相关的信号中发挥作用。
Zebrafish larvae imprint on visual and olfactory kin cues at day 5 and 6 postfertilization, respectively, resulting in kin recognition later in life. Exposure to non-kin cues prevents imprinting and kin recognition. Imprinting depends on MHC class II related signals and only larvae sharing MHC class II alleles can imprint on each other. Here, we analyzed which type of olfactory sensory neuron (OSN) detects kin odor. The single teleost olfactory epithelium harbors ciliated OSNs carrying OR and TAAR gene family receptors (mammals: main olfactory epithelium) and microvillous OSNs with V1R and V2R gene family receptors (mammals: vomeronasal organ). Additionally, teleosts exhibit crypt cells which possess microvilli and cilia. We used the activity marker pERK (phosphorylated extracellular signal regulated kinase) after stimulating 9 day old zebrafish larvae with either non-kin conspecific or food odor. While food odor activated both ciliated and microvillous OSNs, only the latter were activated by conspecific odor, crypt cells showed no activation to both stimuli. Then, we tested imprinted and non-imprinted larvae (full siblings) for kin odor detection. We provide the first direct evidence that crypt cells, and likely a subpopulation of microvillous OSNs, but not ciliated OSNs, play a role in detecting a kin odor related signal.