The reactive site of marinostatin, a proteinase inhibitor from marine Alteromonas sp. B-10-31.
The reactive site of marinostatin, a proteinase inhibitor from marine Alteromonas sp. B-10-31.
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马林抑素（一种来自海洋交替单胞菌的蛋白酶抑制剂）的反应位点。
DOI：
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发表时间：
1991
期刊：
影响因子：
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通讯作者：
S. Hara
中科院分区：
文献类型：
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作者：
R. Takano;C. Imada;K. Kamei;S. Hara
从海洋交替单胞菌（Alteromonas sp.）B-10-31中分离到一个新的肽类蛋白酶抑制剂marinostatin的同系物，命名为marinostatin D。其氨基酸序列为Ala-Thr-Met-Arg-Tyr-Pro-Ser-Asp-Asp-Ser-Glu。根据TLCK-胰凝乳蛋白酶催化可裂键的可逆断裂和再生，确定marinostatin D的反应位点为Met（3）-Arg（4）。
A new homologue of marinostatin, a peptide proteinase inhibitor, was isolated from marine Alteromonas sp. B-10-31 and designated as marinostatin D. Its amino acid sequence was determined to be Ala-Thr-Met-Arg-Tyr-Pro-Ser-Asp-Asp-Ser-Glu. The reactive site of marinostatin D was determined to be Met(3)-Arg(4) on the basis of the reversible cleavage and regeneration of the scissile bond catalyzed by TLCK-chymotrypsin.