Chemistry Is Dead. Long Live Chemistry!
Chemistry Is Dead. Long Live Chemistry!
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DOI：
10.1021/acs.biochem.7b00529
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发表时间：
2017-10-03
期刊：
影响因子：
2.9
通讯作者：
Lavis, Luke D.
中科院分区：
文献类型：
--
作者：
Lavis, Luke D.
化学，曾经的荧光显微镜之王，被荧光蛋白质领域所取代。现代显微技术对“光子预算”的需求日益增加，需要更好、更明亮的荧光团，这引起了人们对合成染料的新兴趣。在这里，我们回顾了生物化学、蛋白质工程和有机合成方面的最新进展，这些进展使化学荧光团成功地重返现代生物成像。
Chemistry, once king of fluorescence microscopy, was usurped by the field of fluorescent proteins. The increased demands of modern microscopy techniques on the "photon budget" require better and brighter fluorophores, causing a renewed interest in synthetic dyes. Here, we review the recent advances in biochemistry, protein engineering, and organic synthesis that have allowed a triumphant return of chemical fluorophores to modern biological imaging.