INTERFERON-GAMMA AND INTERLEUKIN-L-BETA INDUCE NITRIC-OXIDE FORMATION FROM PRIMARY MOUSE ASTROCYTES
INTERFERON-GAMMA AND INTERLEUKIN-L-BETA INDUCE NITRIC-OXIDE FORMATION FROM PRIMARY MOUSE ASTROCYTES
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DOI：
10.1016/0304-3940(93)90898-u
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发表时间：
1993-12-24
影响因子：
2.5
通讯作者：
CHOI, DW
中科院分区：
文献类型：
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作者：
HEWETT, SJ;CORBETT, JA;CHOI, DW
在某些细胞类型中存在一种能够产生大量一氧化氮（NO）的诱导型一氧化氮合酶（iNOS）。我们通过免疫沉淀和亚硝酸盐的形成证明，白细胞介素-1 β (Il1 β)和干扰素- γ (INF γ)在小鼠皮质星形胶质细胞原代培养中诱导一氧化氮合酶的表达。这种诱导具有时间和剂量依赖性，可被NOS抑制剂n - g -硝基- l -精氨酸和蛋白质合成抑制剂环己亚胺抑制。
An inducible form of nitric oxide synthase (iNOS) capable of producing large quantities of nitric oxide (NO) exists in some cell types. We demonstrate by immunoprecipitation and nitrite formation that interleukin-1 beta (Il1 beta) plus interferon-gamma (INF gamma) induce the expression of nitric oxide synthase in primary cultures of murine cortical astrocytes. This induction is time and dose dependent, and inhibited by the NOS inhibitor N-G-nitro-L-arginine and the protein synthesis inhibitor cycloheximide.