Seasonal induced changes in spinach rhizosphere microbial community structure with varying salinity and drought
Seasonal induced changes in spinach rhizosphere microbial community structure with varying salinity and drought
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DOI：
10.1016/j.scitotenv.2016.11.151
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发表时间：
2017-02-01
影响因子：
9.8
通讯作者：
Suarez, Donald L.
中科院分区：
文献类型：
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作者：
Ibekwe, A. Mark;Ors, Selda;Suarez, Donald L.
盐分是灌溉农业中的常见问题，特别是在美国西南部的低降雨量和高蒸发需求地区以及世界各地的其他半干旱地区。然而，盐分对土壤微生物群落的影响的研究相对较少，而灌溉引起的盐分对土壤化学和物理性质以及植物生长的影响则有很好的记录。在这项研究中，我们研究了盐度，温度和时间变化对土壤和根际微生物群落的影响，在砂缸灌溉准备的解决方案，旨在模拟含盐废水。用菠菜（Spinacia oleracea L.，CV.浣熊）进行了盐水在不同的时间段（初冬，春末，初夏）。使用MiSeq（R）Illumina测序平台，利用针对周围保守区域设计的融合引物扩增细菌16 S V4 rDNA区域。在这两种样品类型中，细菌相对占主导地位的三门变形菌，蓝藻，拟杆菌-占77.1%的类群在根际中检测到，而变形菌，拟杆菌，放线菌占55.1%的类群在土壤中检测到。采用UniFrac结合主坐标分析（PCoA）对结果进行分析，以比较土壤和根际样品中的多样性、丰度、群落结构和特定细菌群。方差分析结果表明，土壤温度（P = 0.001）、根际温度（P = 0.001）、根际盐分（P = 0.032）和蒸散量（P = 0.002）对土壤和根际微生物群落β多样性有显著影响。此外，盐分对土壤β多样性的影响很小（P = 0.078）。然而，时间的变化差异影响根际微生物群落灌溉含盐废水。因此，受咸水灌溉水影响的土壤微生物群落对灌溉水质和应用季节的反应不同，这是由于与温度相关的时间效应。由爱思唯尔公司出版
Salinity is a common problem under irrigated agriculture, especially in low rainfall and high evaporative demand areas of southwestern United States and other semi-arid regions around the world. However, studies on salinity effects on soil microbial communities are relatively few while the effects of irrigation-induced salinity on soil chemical and physical properties and plant growth are well documented. In this study, we examined the effects of salinity, temperature, and temporal variability on soil and rhizosphere microbial communities in sand tanks irrigated with prepared solutions designed to simulate saline wastewater. Three sets of experiments with spinach (Spinacia oleracea L., cv. Racoon) were conducted under saline water during different time periods (early winter, late spring, and early summer). Bacterial 16S V4 rDNA region was amplified utilizing fusion primers designed against the surrounding conserved regions using MiSeq (R) Illumina sequencing platform. Across the two sample types, bacteria were relatively dominant among three phyla-the Proteobacteria, Cyanobacteria, and Bacteroidetes-accounted for 77.1% of taxa detected in the rhizosphere, while Proteobacteria, Bacteroidetes, and Actinobacteria accounted for 55.1% of taxa detected in soil. The results were analyzed using UniFrac coupled with principal coordinate analysis (PCoA) to compare diversity, abundance, community structure, and specific bacterial groups in soil and rhizosphere samples. Permutational analysis of variance (PERMANOVA) analysis showed that soil temperature (P = 0.001), rhizosphere temperature (P = 0.001), rhizosphere salinity (P = 0.032), and evapotranspiration (P = 0.002) significantly affected beta diversity of soil and rhizosphere microbial communities. Furthermore, salinity had marginal effects (P = 0.078) on soil beta diversity. However, temporal variability differentially affected rhizosphere microbial communities irrigated with saline wastewater. Therefore, microbial communities in soils impacted by saline irrigation water respond differently to irrigation water quality and season of application due to temporal effects associated with temperature. Published by Elsevier B.V.