Horizontal gene transfer contributes to virulence and antibiotic resistance of Vibrio harveyi 345 based on complete genome sequence analysis
Horizontal gene transfer contributes to virulence and antibiotic resistance of Vibrio harveyi 345 based on complete genome sequence analysis
复制标题
基于完整基因组序列分析，水平基因转移有助于哈维氏弧菌 345 的毒力和抗生素耐药性
DOI：
10.1186/s12864-019-6137-8
复制
发表时间：
2019-10-22
期刊：
影响因子：
4.4
通讯作者：
Feng, Juan
中科院分区：
文献类型：
--
作者：
Deng, Yiqin;Xu, Haidong;Feng, Juan
背景
水平基因转移（HGT）受环境污染和气候变化影响，促进遗传通讯，改变细菌致病性和耐药性。然而，关于HGT对机会致病菌哈维氏弧菌高致病性和耐药性的影响的研究还很少。
结果
V. harveyi 345 具有多重耐药性并感染了斑斑石斑鱼，是从中国南部深圳（一个重要的污染水产养殖区）的患病生物中分离出来的。对整个基因组序列的分析预测了 5678 个基因，其中包括 487 个导致细菌发病机制的毒力基因和 25 个导致抗菌药物耐药性的抗生素耐药基因 (ARG)。 pAQU 型质粒 p345-185 上的 5 个 ARG（tetm、tetb、qnrs、dfra17 和 sul2）和 1 个毒力基因 (CU052_28670) 为 HGT 提供了直接证据。对 31 个哈维氏弧菌菌株的比较基因组分析表明，217 个基因和 7 个基因家族，包括 C 类 β-内酰胺酶基因、毒力相关蛋白 D 基因和 OmpA 家族蛋白基因是菌株 V. harveyi 345 特有的。这些基因可能有助于 HGT 或从其他细菌水平转移，以增强 345 的毒力或抗生素抗性。71 个基因组岛中的可移动遗传元件编码毒力因子检测到3个III型分泌蛋白和13个VI型分泌系统蛋白，以及两个不完整的原噬菌体序列，可能是HGT转移工具。对V. harveyi 345全基因组的评估和比较基因组学表明存在基因组交换，特别是HGT引起的致病基因和耐药基因的交换，有助于致病性和耐药性。气候变化和持续的环境恶化预计将加速哈维弧菌的 HGT，增加其致病性和耐药性。
结论
这项研究为进一步分析哈维氏弧菌发病机制和抗菌素耐药性以及制定沿海地区污染控制措施提供了及时的信息。
Background
Horizontal gene transfer (HGT), which is affected by environmental pollution and climate change, promotes genetic communication, changing bacterial pathogenicity and drug resistance. However, few studies have been conducted on the effect of HGT on the high pathogenicity and drug resistance of the opportunistic pathogen Vibrio harveyi.
Results
V. harveyi 345 that was multidrug resistant and infected Epinephelus oanceolutus was isolated from a diseased organism in Shenzhen, Southern China, an important and contaminated aquaculture area. Analysis of the entire genome sequence predicted 5678 genes including 487 virulence genes contributing to bacterial pathogenesis and 25 antibiotic-resistance genes (ARGs) contributing to antimicrobial resistance. Five ARGs (tetm, tetb, qnrs, dfra17, and sul2) and one virulence gene (CU052_28670) on the pAQU-type plasmid p345–185, provided direct evidence for HGT. Comparative genome analysis of 31 V. harveyi strains indicated that 217 genes and 7 gene families, including a class C beta-lactamase gene, a virulence-associated protein D gene, and an OmpA family protein gene were specific to strain V. harveyi 345. These genes could contribute to HGT or be horizontally transferred from other bacteria to enhance the virulence or antibiotic resistance of 345. Mobile genetic elements in 71 genomic islands encoding virulence factors for three type III secretion proteins and 13 type VI secretion system proteins, and two incomplete prophage sequences were detected that could be HGT transfer tools. Evaluation of the complete genome of V. harveyi 345 and comparative genomics indicated genomic exchange, especially exchange of pathogenic genes and drug-resistance genes by HGT contributing to pathogenicity and drug resistance. Climate change and continued environmental deterioration are expected to accelerate the HGT of V. harveyi, increasing its pathogenicity and drug resistance.
Conclusion
This study provides timely information for further analysis of V. harveyi pathogenesis and antimicrobial resistance and developing pollution control measurements for coastal areas.