A MOUSE HOMOLOG OF THE ESCHERICHIA-COLI RECA AND SACCHAROMYCES-CEREVISIAE RAD51 GENES
A MOUSE HOMOLOG OF THE ESCHERICHIA-COLI RECA AND SACCHAROMYCES-CEREVISIAE RAD51 GENES
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DOI：
10.1073/pnas.90.14.6577
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发表时间：
1993-07-15
影响因子：
11.1
通讯作者：
MATSUSHIRO, A
中科院分区：
文献类型：
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作者：
MORITA, T;YOSHIMURA, Y;MATSUSHIRO, A
哺乳动物细胞中有丝分裂和减数分裂重组的分析由于涉及的反应的复杂性以及缺乏突变体而受到阻碍。此外，参与这一过程的基因还没有被识别出来。在萌芽酵母中，RAD51基因和RAD52上位组的其他基因对于有丝分裂和减数分裂重组和DNA修复是必不可少的。RAD51蛋白在结构上与大肠杆菌RecA蛋白相似，后者是细菌中同源重组和SOS反应所必需的。在这里，我们报告了酵母RAD51基因的一个小鼠同源基因的分离。该基因预测的氨基酸序列与酵母RAD51和大肠杆菌recA产物的氨基酸序列分别有83%和55%的同源性。小鼠的基因补充了酿酒酵母的RAD51突变，对甲基甲烷磺酸盐敏感，甲基甲烷磺酸盐会产生DNA双链断裂。该基因在胸腺、睾丸、卵巢、脾和肠道中表达，表明其产物除了参与DNA修复外，还参与有丝分裂和减数分裂重组。
Analysis of mitotic and meiotic recombination in mammalian cells has been hampered by the complexity of the reactions involved as well as lack of mutants. Furthermore, none of the genes involved in the process has yet been identified. In budding yeast, Saccharomyces cerevisiae, the RAD51 gene is essential along with other genes of the RAD52 epistasis group for mitotic and meiotic recombination and DNA repair. The Rad51 protein is structurally similar to Escherichia coli RecA protein, which is required in homologous recombination and SOS responses in bacteria. Here we report the isolation of a mouse homolog of the yeast RAD51 gene. The amino acid sequence predicted from the gene shows 83% and 55% homology with those of the yeast RAD51 and the E. coli recA product, respectively. The mouse gene complemented a rad51 mutation of S. cerevisiae with sensitivity to methylmethanesulfonate, which produces double-strand breaks of DNA. This gene is expressed in the thymus, testis, ovary, spleen, and intestine, suggesting that its product is involved in mitotic and meiotic recombination in addition to DNA repair.