PetM Is Essential for the Stabilization and Function of the Cytochrome b 6 f Complex in Arabidopsis
PetM Is Essential for the Stabilization and Function of the Cytochrome b 6 f Complex in Arabidopsis
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DOI：
10.1093/pcp/pcab116
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发表时间：
2021
期刊：
影响因子：
--
通讯作者：
Hualing Mi
中科院分区：
文献类型：
--
作者：
Yixin Lan;Qi Chen;Mengmeng Kong;Yanyun Liu;Lyu Ming-Ju Amy;Perveen Shahnaz;Hualing Mi
The cytochrome b6f (cyt b6f) acts as a center linker of electron transport between photosystem I and II in oxygenic photosynthesis. PetM, one of the eight subunits of the cyt b6f complex, is a small hydrophobic subunit at the outside periphery, the functional mechanism of which remains to be elucidated in higher plants. In this work, we found that unlike the PetM mutant in Synechocystis sp. PCC 6803, the Arabidopsis thaliana PetM mutant showed a bleached phenotype with yellowish leaves, block of photosynthetic electron transport and loss of photo-autotrophy, similar to the Arabidopsis PetC mutant. Although PetM is relatively conserved between higher plants and cyanobacteria, Synechocystis PetM could not rescue the PetM-knockout phenotype in Arabidopsis. We provide evidence that the Synechocystis PetM did not stably bind to the Arabidopsis cyt b6f complex. Based on these results, we suggest that PetM is required in Arabidopsis to maintain function of the cyt b6f complex, likely through its close link with core subunits to form a tight “fence” which stabilizes the core of the complex.
