Up-regulation of macrophage migration-inhibitory factor expression after compression-induced spinal cord injury in rats
Up-regulation of macrophage migration-inhibitory factor expression after compression-induced spinal cord injury in rats
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DOI：
10.1007/s00401-004-0853-z
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发表时间：
2004-07-01
影响因子：
12.7
通讯作者：
Yamazaki, M
中科院分区：
文献类型：
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作者：
Koda, M;Nishio, Y;Yamazaki, M
巨噬细胞迁移抑制因子（Macrophage migration inhibitory factor， MIF）是一种多电位蛋白，具有促炎细胞因子、垂体激素、免疫调节剂和丝裂原的作用。为了阐明MIF在脊髓损伤中的作用，我们采用Northern blot分析、原位杂交和免疫组织化学方法检测了脊髓受压损伤后MIF的表达。Northern blot分析显示，损伤脊髓中MIF mRNA表达上调，在损伤后3天达到峰值。原位杂交显示损伤后3天病变中心聚集的小胶质细胞和损伤后1周囊腔周围星形胶质细胞的MIF上调。双染色显示MIF和番茄凝集素在病变部位共定位，表明积聚到病变中心的小胶质细胞表达MIF。MIF表达的时间过程与以往报道的细胞因子表达高峰出现在更早的时间点不同；因此，在本研究中，MIF不太可能作为促炎因子。由于其促进细胞增殖的特性，MIF可能有助于脊髓损伤部位周围星形胶质细胞的增殖。
Macrophage migration inhibitory factor (MIF) is a multipotential protein that acts as a pro-inflammatory cytokine, pituitary hormone, immunoregulator, and mitogen. To elucidate function of MIF in spinal cord injury, we examined expression of MIF after compression-induced spinal cord injury using Northern blot analysis, in situ hybridization and immunohistochemistry. The MIF mRNA was up-regulated in injured spinal cord, peaking 3 days after injury shown by Northern blot analysis. In situ hybridization revealed up-regulation of MIF in microglia accumulating in the lesion epicenter 3 days after injury and astrocytes around the cystic cavity 1 week after injury. Double staining showed co-localization of MIF and tomato lectin in the lesioned site, indicating that microglia accumulating to the lesion epicenter express MIF. The time course of MIF expression is different from that of previous reports about cytokine expression peaking at earlier time points; thus, it is unlikely that MIF acts as a pro-inflammatory factor in the present study. The MIF may contribute to proliferation of astrocytes around the lesioned site in spinal cord injury because of its cell proliferation-promoting property.