Development of a rat model of bisphosphonate-related osteonecrosis of the jaw (BRONJ).
Development of a rat model of bisphosphonate-related osteonecrosis of the jaw (BRONJ).
复制标题
双膦酸盐相关颌骨坏死大鼠模型的开发（BRONJ）。
DOI：
--
复制
发表时间：
2012
影响因子：
1.6
通讯作者：
J. Borke
中科院分区：
文献类型：
--
作者：
Karen L. Marino;Ibrahim E. Zakhary;R. Abdelsayed;Jared A Carter;J. C. O'Neill;R. Khashaba;M. Elsalanty;M. Stevens;J. Borke
本研究的目的是建立一个大鼠模型，预测拔牙后双膦酸盐相关的颌骨骨坏死（BRONJ）。将30只雌性大鼠随机分为对照组和实验组。实验组静脉注射唑来膦酸20 μg/kg，共2次。拔除右下颌第一磨牙近中牙根。在第0、4和8周对大鼠实施安乐死。检查骨密度（BMD）、胶原分解（吡啶[PYD]）、血管再生（VEGF）和组织学。在对照组和实验组中，伤后PYD值有升高的趋势。对照组和实验组伤后血清VEGF均显著升高。8周后，仅实验组VEGF继续升高。在拔牙窝区域，对照组与唑来膦酸治疗组大鼠受伤后BMD显著降低。实验组的组织学切片显示细菌和骨坏死。在唑来膦酸盐治疗后观察到与人类相似的BRONJ特征的一致结果。
The purpose of this study was to develop a rat model predictive of bisphosphonate-related osteonecrosis of the jaw (BRONJ) after exodontias. Thirty female rats were randomized into 2 groups, control and experimental. The experimental group received 2 intravenous injections of zoledronate (20 μg/kg). The mesial root of the right mandibular first molar was extracted. Rats were euthanized at 0, 4, and 8 weeks. Bone mineral density (BMD), collagen breakdown (pyridinium [PYD]), vascular regeneration (VEGF), and histology were examined. A trend toward higher PYD values was suggested in control vs experimental groups after wounding. Serum VEGF increased significantly after wounding for both control and experimental groups. After 8 weeks, VEGF continued to rise for the experimental group only. In the extraction socket area, BMD was significantly lower after wounding in control vs. zoledronate-treated rats. Histology sections from experimental groups showed bacteria and bone necrosis. Consistent findings of BRONJ features similar to those in humans were observed after zoledronate treatment.