Efficient systemic DNA delivery to the tumor by self-assembled nanoparticle
Efficient systemic DNA delivery to the tumor by self-assembled nanoparticle
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DOI：
10.1007/s11051-013-2213-1
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发表时间：
2013
影响因子：
2.5
通讯作者：
Hailin Tang;Xinhua Xie;Jiaoli Guo;Weidong Wei;Minqing Wu;Peng Liu;Y. Kong;Lu Yang;M. Hung;Xiaoming Xie
中科院分区：
文献类型：
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作者：
Hailin Tang;Xinhua Xie;Jiaoli Guo;Weidong Wei;Minqing Wu;Peng Liu;Y. Kong;Lu Yang;M. Hung;Xiaoming Xie
目前能高效低毒传递基因的递送剂很少，尤其是动物实验。因此，构建具有良好投递效率和安全性的载体是一项有意义的工作。我们开发了一种自组装基因递送系统(XM001)，与商业递送剂相比，它可以更有效地将DNA递送到多个细胞系和乳腺肿瘤。此外，全身给药XM001-BikDD(BikDD是促凋亡基因Bik的突变形式)显著抑制了人乳腺癌细胞的生长，并延长了裸鼠移植瘤的寿命。本研究表明，XM001是一种高效、广泛的转染剂，有望成为肿瘤靶向治疗的肿瘤载体。
There are few delivery agents that could deliver gene with high efficiency and low toxicity, especially for animal experiments. Therefore, creating vectors with good delivery efficiency and safety profile is a meaningful work. We have developed a self-assembled gene delivery system (XM001), which can more efficiently deliver DNA to multiple cell lines and breast tumor, as compared to commercial delivery agents. In addition, systemically administrated XM001–BikDD (BikDD is a mutant form of proapoptotic gene Bik) significantly inhibited the growth of human breast cancer cells and prolonged the life span in implanted nude mice. This study demonstrates that XM001 is an efficient and widespread transfection agent, which could be a promising tumor delivery vector for cancer targeted therapy.