Molecular markers closely linked to the dominant male sterility gene CDMs399-3 in Brassica oleracea.
Molecular markers closely linked to the dominant male sterility gene CDMs399-3 in Brassica oleracea.
复制标题
DOI：
--
复制
发表时间：
2009
期刊：
影响因子：
--
通讯作者：
Zhang Xinmei;Wu Jian;Guo Aiguang;Z. Hui;Fang Zhiyuan;Wang Xiaowu
中科院分区：
文献类型：
--
作者：
Zhang Xinmei;Wu Jian;Guo Aiguang;Z. Hui;Fang Zhiyuan;Wang Xiaowu
【目的】筛选与甘蓝CDMs 399 -3基因连锁的分子标记。【方法】以CDMs 399 -3为材料，利用SRAP和SSR技术，在BSA法的基础上，对CDMs 399 -3进行分子标记分析。【结果】共检测到8个SRAP标记，1个SSR标记和1个SCAR标记与CDMs 399 - 3紧密连锁，其中2个侧翼标记ENA 14 F-CoEm 7 R和8 C 0909分别与CDMs 399 -3连锁，距离分别为0.53 cM和2.55 cM，ENA 14 F-CoEm 7 R转化为序列特征扩增区（SCAR）标记。【结论】该标记为CDMs 399 -3基因的精细定位奠定了基础，可用于雄性不育基因的精确标记辅助选择。
【Objective】In this study,molecular markers linked to CDMs399-3 gene in Brassica oleracea were screened.【Method】 SRAP and SSR technologies were used to identify markers linked to CDMs399-3 based on BSA method.The bulks were formed using malesterile and malefertile individuals from a segregating BC4 population.【Result】 A total of 8 SRAP were identified,1 SSR and 1 SCAR markers closely linked to the male sterility gene.The two flanking markers,ENA14F-CoEm7R and 8C0909,were linked to the CDMs399-3 with a distance of 0.53 cM and 2.55 cM,respectively.ENA14F-CoEm7R was converted into a sequence characterized amplified region(SCAR) marker.【Conclusion】 The markers developed here founded a solid basis for fining mapping of CDMs399-3 gene and were useful for accurate marker-assisted selection(MAS) of the male sterility gene.