A SIMPLIFIED STRATEGY FOR SEQUENCE ANALYSIS OF LARGE PROTEINS
A SIMPLIFIED STRATEGY FOR SEQUENCE ANALYSIS OF LARGE PROTEINS
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DOI：
10.1038/193241a0
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发表时间：
1962-01-01
期刊：
影响因子：
64.8
通讯作者：
ECK, RV
中科院分区：
文献类型：
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作者：
ECK, RV
理论上看来比通常的逐步确定氨基酸序列的方法要有效得多的策略，已经在一个400个连接的“蛋白质”的“纸上实验”中得到了检验。这种策略在蛋白质密码的准备工作中就出现了。在汇编已发表的氨基酸序列数据时，通常不试图评价每个数据的相对可靠性。相反，密码计算是以这样一种方式计划的，即数据中偶尔的错误是可以容忍的。然而，溶菌酶的结构是个例外，两个实验室报告了大量的结果，每个实验室几乎都占了所有的经验公式，结果显示出相当广泛的分歧。Jollès和他的学校使用了各种酶和对原始蛋白质的特殊处理。完整的蛋白质（132个链接）被认为太大而无法直接分析，因此使用酶将其还原为中等大小的链，然后通过酸水解和其他方法分离，纯化和分析。大约有一半的联系是最终确定的，其余的大部分由酶消化的大产物的组成分析表示。
STRATEGY which appears theoretically much more efficient than the usual stepwise procedures for determining amino-acid sequences has been tested in a “paper experiment” with a “protein” of 400 links. This strategy suggested itself during work in preparation for the protein cryptogram**. In compiling published data on amino-acid sequences, no attempt was usually made to evaluate the 1 elative reliability of each datum. Instead, the cryptogram calculations were planned in such a way that occasional errors in the data could be tolerated. However, an exception had to be made in the case of the structuro of lysozyme.Two laboratories had reported voluminous results, each accounting for almost all the empirical formula, which showed quite extensive disagreement. Jollès and his school had used various enzymes and special treatments of the original protein. The intact protein (132 links) was considered to be too large for direct analysis, so enzymes were used to reduce it to medium-sized chains, which were then separated, purified, and analysed by acid hydrolysis and other methods. About half the linkages wero finally identified, and most of the remainder represented by composition analyses of large products of enzyme digestion".