Salvia miltiorrhiza inhibits cell growth and induces apoptosis in human hepatoma HepG2 cells
Salvia miltiorrhiza inhibits cell growth and induces apoptosis in human hepatoma HepG2 cells
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DOI：
10.1016/s0304-3835(00)00391-8
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发表时间：
2000-05-29
期刊：
影响因子：
9.7
通讯作者：
Ong, CN
中科院分区：
文献类型：
--
作者：
Liu, J;Shen, HM;Ong, CN
丹参（SM）是一种传统中草药，几个世纪以来在中国常用来治疗肝病。一些早期研究表明 SM 具有抗肿瘤特性，但其机制仍有待阐明。在本研究中，我们评估了 SM 在人肝癌细胞系 HepG(2) 中的分子机制。我们的结果表明SM具有明显的细胞毒作用，并强烈抑制HepG(2)细胞的增殖。还观察到 SM 处理引起凋亡细胞死亡，如下评估：(a)，使用吖啶橙/溴化乙锭染色进行形态学变化； (b)，通过 TdT 介导的 dUTP 缺口末端标记 (TUNEL) 进行 DNA 片段化； (c)，亚G(1)细胞分析。此外，细胞内谷胱甘肽 (GSH) 的消耗和线粒体膜电位的降低被发现与 SM 启动细胞凋亡有关。 (C) 2000 Elsevier Science Ireland Ltd. 保留所有权利。
Salvia miltiorrhiza (SM) is a traditional Chinese herbal medicine, commonly used to treat liver diseases in China for centuries. Several earlier studies have indicated that SM exhibits anti-tumor properties, but its mechanism remains to be elucidated. In this study, we evaluated the molecular mechanism of SM in a human hepatoma cell line, HepG(2). Our results show that SM exerted clear cytotoxic effects, and strongly inhibited the proliferation of HepG(2) cells. It was also observed that SM treatment caused apoptotic cell death as evaluated by: (a), morphological changes by using acridine orange/ethidium bromide staining; (b), DNA fragmentation by TdT-mediated dUTP nick end labeling (TUNEL); and (c), sub-G(1) cell analysis. Furthermore, depletion of intracellular glutathione (GSH) and reduction of mitochondrial membrane potential were found to be involved in the initiation of apoptosis by SM. (C) 2000 Elsevier Science Ireland Ltd. All rights reserved.