Functional Responses of Human Adipose Tissue-Derived Mesenchymal Stem Cells to Metal Oxide Nanoparticles In Vitro
Functional Responses of Human Adipose Tissue-Derived Mesenchymal Stem Cells to Metal Oxide Nanoparticles In Vitro
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DOI：
10.1166/jbn.2013.1473
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发表时间：
2013-01-01
影响因子：
2.9
通讯作者：
Kleinsasser, Norbert
中科院分区：
文献类型：
--
作者：
Hackenberg, Stephan;Scherzed, Agmal;Kleinsasser, Norbert
纳米颗粒（NP）经常应用于生物医学应用。人类间充质干细胞（hMSC）在生物医学中的应用至关重要，特别是在肿瘤学和组织工程中。二氧化钛 (TiO2) 和氧化锌 (ZnO) 纳米颗粒是实验肿瘤学中令人感兴趣的试剂，干细胞被认为是纳米颗粒到达肿瘤部位的潜在载体。然而，人们对 NPs 对 hMSC 的危险作用知之甚少。本研究的目的是分析 ZnO-和 TiO2-NPs 对 hMSC 的功能损伤。通过 MTT 测定评估 NP 的细胞毒性作用。此外，还评估了多分化能力、球体形成和迁移。通过流式细胞术观察免疫表型。在 625 nM ZnO-NP 下观察到细胞毒性作用，而 TiO2-NP 在 hMSC 中未观察到细胞毒性。 hMSC 分化成成骨和脂肪谱系的能力没有改变。暴露于 NP 后对 hMSC 进行 3 周的长期培养，结果显示 NP 在细胞质中持续存在。迁移能力受损，但形成球体的能力不受影响。流式细胞术分析显示细胞表面标志物 CD 90 和 CD 73 发生明显变化。hMSC 的主要功能特性不受 TiO2- 和 ZnO-NP 的影响。然而，限制迁移可能会严重影响伤口愈合能力。需要更多信息来评估这些发现的临床影响。
Nanoparticles (NPs) are frequently applied in biomedical applications. The use of human mesenchymal stem cells (hMSC) in biomedicine is pivotal, especially in oncology and tissue engineering. Titanium dioxide (TiO2) and zinc oxide (ZnO) NPs are interesting agents in experimental oncology and stem cells are discussed to be a potential vehicle for NPs to tumor sites. However, little is known about hazardous effects of NPs in hMSC. The aim of the present study was to analyze functional impairment of hMSC by ZnO- and TiO2-NPs. Cytotoxic effects of NPs were evaluated by the MTT-assay. Furthermore, multi-differentiation capacity, spheroid formation and migration were assessed. The immunophenotype was observed by flow cytometry. Cytotoxic effects were observed at 625 nM ZnO-NPs whereas no cytotoxicity was seen in hMSC by TiO2-NPs. The differentiation capacity of hMSC into osteogenic and adipose lineages was unchanged. A long-term period cultivation of hMSC for 3 weeks after NP exposure revealed a persistence of NPs in the cytoplasm. The migration capability was impaired whereas the ability to form spheroids was not affected. Flow cytometric analysis revealed distinct alteration of cell surface markers CD 90 and CD 73. Major functional properties of hMSC were unaffected by TiO2- and ZnO-NPs. However, restricted migration might critically influence wound healing capacity. Further information is needed to assess the clinical impact of these findings.