A microsatellite polymorphism in the gamma interferon gene is associated with resistance to gastrointestinal nematodes in a naturally-parasitized population of Soay sheep
A microsatellite polymorphism in the gamma interferon gene is associated with resistance to gastrointestinal nematodes in a naturally-parasitized population of Soay sheep
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DOI：
10.1017/s0031182001007570
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发表时间：
2001-05-01
期刊：
影响因子：
2.4
通讯作者：
Pemberton, JM
中科院分区：
文献类型：
--
作者：
Coltman, DW;Wilson, K;Pemberton, JM
自由生活的Soay羊（Ovis aries）在希尔塔岛，圣基尔达，苏格兰，自然寄生bq胃肠道线虫，主要是Teladorsagia circumcincta。在本文中，我们表明，减少粪便鸡蛋计数（FEC）与等位基因位于第一内含子的干扰素γ基因（o（IFN）-γ）在Soay绵羊羔羊和一岁，测量约4个月和16个月的年龄，分别在微卫星位点。同一等位基因也与T.羔羊的包皮环切特异性抗体（伊加），但不相关的一岁的显着。侧翼对照标记未能显示;与FEC或伊加显著相关。这些结果表明，一个多态性基因，赋予增加抵抗胃肠道线虫寄生虫位于或附近的干扰素γ基因，并支持以前的报告，已映射的数量性状位点（QTL）的抗性，这一地区在国内绵羊。我们的数据是一致的想法，导致（IFN）-γ的表达或疗效降低的功能性多态性，可以通过有利于Th-2细胞亚群的活性和抗体相关的免疫机制，增强对胃肠道线虫的免疫应答。
Free-living Soay sheep (Ovis aries) on the island of Hirta, St Kilda, Scotland, are naturally parasitized bq gastrointestinal nematodes, predominantly Teladorsagia circumcincta. In this paper we show that reduced faecal egg counts (FEC) are associated with an allele at a microsatellite locus located in the first intron of the interferon gamma gene (o(IFN)-gamma) in Soay sheep lambs and yearlings, measured at approximately 4 and 16 months of age, respectively. The same allele nas also associated with increased T. circumcincta-specific antibody (IgA) in lambs, but not associated significantly in yearlings. Flanking control markers failed to show; a significant association with either FEC or IgA. These results suggest that a polymorphic gene conferring increased resistance to gastrointestinal nematode parasites is located at or near the interferon gamma gene, and support previous reports which have mapped a quantitative trait locus (QTL) for resistance to this region in domestic sheep. Our data are consistent with the idea that a functional polymorphism leading to reduced expression or efficacy of (IFN)-gamma could enhance the immune response to gastrointestinal nematodes by favouring the activity of the Th-2 cell subset and antibody associated immune mechanisms.