Comparison between proteinases of human seminal plasma and of sperm origin.
Comparison between proteinases of human seminal plasma and of sperm origin.
复制标题
人精浆和精子来源的蛋白酶之间的比较。
DOI：
10.1002/j.1939-4640.1987.tb03309.x
复制
发表时间：
1987
影响因子：
--
通讯作者：
Polakoski,KL
中科院分区：
文献类型：
--
作者：
Hume,ME;Siegel,MS;Polakoski,KL
用明胶-十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法比较人精浆、精液非配子细胞质和精子的碱性蛋白酶活性。在未做输精管结扎术和输精管结扎术的健康供者的精浆样本中，可以看到几条主要的(分子量=56,000)和次要的(35,000到44,000)条蛋白酶活性条带。在输精管结扎术后精液的非配子细胞材料中也观察到了相似的活性特征。精子提取物中的主要蛋白酶活性在47,000-55,000(顶体酶原-顶体酶)和34,000-37,000(精子原-精蛋白)分子量范围内。这些结果表明，原顶体酶-顶体酶和精原-精蛋白系统起源于精子，人精浆的可溶性和非受精性细胞质中存在相当数量的较大分子量的类胰蛋白酶。
A comparison of the alkaline proteinase activity of human seminal plasma, the seminal non‐gamete cellular material and spermatozoa was made by gelatin‐sodium dodecyl sulfate‐polyacrylamide gel electrophoresis (gelatin‐SDS‐PAGE) zymography. Several major (molecular weights = > 56,000) and minor (35,000 to 44,000) bands of proteinase activity were seen in the seminal plasma samples from nonvasectomized and vasectomized, healthy donors. Similar activity profiles were observed in the nongamete cellular material of vasectomized donor ejaculates. The major proteinase activity in sperm extracts was in the 47,000 to 55,000 (proacrosin‐acrosin) and 34,000 to 37,000 (sperminogen‐spermin) molecular weight ranges. These results suggest that the proacrosin‐acrosin and sperminogen‐spermin systems are of sperm origin and that there are considerable amounts of larger molecular weight trypsin‐like enzymes in the soluble and nongamete cellular material of human seminal plasma.