Direct effects of growth hormone on insulin action in rat adipose tissue maintained in vitro.
Direct effects of growth hormone on insulin action in rat adipose tissue maintained in vitro.
复制标题
生长激素对体外维持的大鼠脂肪组织中胰岛素作用的直接影响。
DOI：
10.1210/endo-107-2-538
复制
发表时间：
1980
期刊：
影响因子：
4.8
通讯作者：
Dean H. Lockwood
中科院分区：
文献类型：
--
作者：
B. L. Maloff;Joseph H. Levine;Dean H. Lockwood
以前的研究表明，GH过量状态下胰岛素作用受损。在目前的工作中，脂肪组织的器官培养系统被用来试图表征那些直接归因于GH的致糖尿病细胞改变。在不存在或存在牛或人GH（5.0 × 10-7至5.0 × 10-10 M）的情况下，将大鼠附睾脂肪组织片在补充培养基199中培养20-44 h。[125 I]在从培养组织分离的脂肪细胞中测量胰岛素结合、基础和胰岛素刺激的2-脱氧葡萄糖摄取以及葡萄糖利用。长期暴露于GH不影响胰岛素受体数量或亲和力。相比之下，GH处理的细胞在基础状态下和响应于0.2-40 ng/ml胰岛素时显示出减少的己糖摄取。然而，胰岛素敏感性并没有改变，因为GH治疗组和未治疗组对最大有效浓度胰岛素的增量反应和引起半最大刺激的胰岛素浓度相同。
Previous studies have shown that there is impaired insulin action in states of GH excess. In the present work, an organ culture system for adipose tissue was used in an attempt to characterize those diabetogenic cellular alterations directly attributable to GH. Pieces of rat epididymal fat tissue were maintained in supplemented medium 199 for 20–44 h in the absence or presence of bovine or human GH (5.0 × 10-7 to 5.0 × 10-10 M). [125I]Insulin binding, basal and insulin-stimulated 2-deoxyglucose uptake, and glucose utilization were measured in adipocytes isolated from the cultured tissue. Prolonged exposure to GH did not influence insulin receptor number or affinity. In contrast, GH-treated cells showed reduced hexose uptake in the basal state and in response to 0.2–40 ng/ml insulin. However, insulin sensitivity was not altered, since the incremental response to maximally effective concentrations of insulin and the concentration of insulin causing half-maximal stimulation were the same in GH-treated and un...