Cloned transgenic swine via in vitro production and cryopreservation
Cloned transgenic swine via in vitro production and cryopreservation
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DOI：
10.1095/biolreprod.106.052514
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发表时间：
2006-08-01
影响因子：
3.6
通讯作者：
Prather, Randall S.
中科院分区：
文献类型：
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作者：
Li, Rongfeng;Lai, Liangxue;Prather, Randall S.
众所周知，成功低温保存猪胚胎是非常困难的，而体外生产的胚胎则更加困难。在体外产生的猪胚胎中，第一个可重复的冷冻保存方法是在离心和去除脂质之后。在这里，我们报告了一个类似的适应过程，允许体外产生的体细胞核移植（SCNT）猪胚胎的低温保存。这些胚胎发育到囊胚期并在低温保存中存活。将163个冷冻保存的SCNT胚胎移植到两个代孕母体内产生了10头仔猪。这项技术的应用可能允许克隆的转基因猪胚胎在国内和国际上的移动，储存直到找到合适的替代物，或长期冷冻有价值的遗传。
It has been notoriously difficult to successfully cryopreserve swine embryos, a task that has been even more difficult for in vitro-produced embryos. The first reproducible method of cryopreserving in vivo-produced swine embryos was after centrifugation and removal of the lipids. Here we report the adaptation of a similar process that permits the cryopreservation of in vitro-produced somatic cell nuclear transfer (SCNT) swine embryos. These embryos develop to the blastocyst stage and survive cryopreservation. Transfer of 163 cryopreserved SCNT embryos to two surrogates produced 10 piglets. Application of this technique may permit national and international movement of cloned transgenic swine embryos, storage until a suitable surrogate is available, or the long-term frozen storage of valuable genetics.