SPREADING OF FIBROBLASTS IN MEDIUM CONTAINING CYTOCHALASIN-B - FORMATION OF LAMELLAR CYTOPLASM AS A COMBINATION OF SEVERAL FUNCTIONALLY DIFFERENT PROCESSES
SPREADING OF FIBROBLASTS IN MEDIUM CONTAINING CYTOCHALASIN-B - FORMATION OF LAMELLAR CYTOPLASM AS A COMBINATION OF SEVERAL FUNCTIONALLY DIFFERENT PROCESSES
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DOI：
10.1073/pnas.77.10.5919
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发表时间：
1980-01-01
期刊：
影响因子：
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通讯作者：
GELFAND, JM
中科院分区：
文献类型：
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作者：
BLIOKH, ZL;DOMNINA, LV;GELFAND, JM
正常培养的小鼠成纤维细胞在固体基质上延伸并附着大量伪足；附着的伪足最终形成板层细胞质。在细胞松弛素B(CB)存在的情况下扩散的成纤维细胞形成新生的树枝状分支突起系统，而不是板层细胞质。与正常的板层细胞质相比，生长且形成完整的乔木状突起具有低的收缩能力，不能从其表面清除打补丁的刀豆蛋白A受体；它们的附着位置与微丝束无关。在含有CB和Colcemid的培养基中展开的细胞没有形成组织良好的分枝结构，而是延伸并附着许多不稳定的伪足。板层细胞质的正常形成可以认为是几个不同功能过程的组合：对CB和Colcemid耐药的基本伪足反应，CB敏感的片段化和Colcemid敏感的稳定。
Normal cultured mouse fibroblasts spreading on solid substrate extend and attach numerous pseudopods; lamellar cytoplasm is eventually formed from the attached pseudopods. Fibroblasts spreading in the presence of cytochalasin B (CB) form de novo a system of arbor-like branched processes rather than lamellar cytoplasm. The growing and fully formed arbor-like processes, in contrast to normal lamellar cytoplasm, have low contractility and are unable to clear patched concanavalin A receptors from their surfaces; their attachment sites are not associated wtih microfilament bundles. The cells spreading in medium containing CB and colcemid do not form well-organized branched structures but extend and attach numerous unstable pseudopods. Normal formation of lamellar cytoplasm can be regarded as a combination of several functionally different processes: rudimentary pseudopodial reactions resistant to CB and colcemid, CB-sensitive lamellization and colcemid-sensitive stabilization.