A, B, H antigen detectability in normal and neoplastic urothelium. Influence of methodologic factors
A, B, H antigen detectability in normal and neoplastic urothelium. Influence of methodologic factors
复制标题
正常和肿瘤性尿路上皮中 A、B、H 抗原的可检测性。
作者：
C. Limas;P. Lange
在新鲜冷冻状态下，无论患者的分泌状态和血型（BG）如何，在正常粘膜中始终可检测到预期抗原，但仅在 69% 的 TCC 中可检测到。石蜡处理后，很少有正常粘膜（12%），但大量TCC（46%）转阴。当用与石蜡程序中使用的浓度相当的酒精处理组织时，也注意到 A、B、H 反应性的类似降低。肿瘤抗原对酒精更敏感。这表明在肿瘤细胞中，ABH 决定簇不仅较少，而且在极性（醇溶性）分子上的比例也不成比例。酒精对非分泌者组织的影响更为深远，可能是因为分泌者和非分泌者中醇溶性 A、B、H 物质的比例不同。
In the fresh‐frozen state, the expected antigen was always detectable in normal mucosas but only in 69% of TCCs regardless of patient's secretor status and blood group (BG). Few normal mucosae (12%) but a large number of TCCs (46%) converted to negative after paraffin processing. Similar reduction in A, B, H reactivity was also noted when the tissues were treated with concentrations of alcohol comparable to those used in the paraffin procedure. Tumor antigens were more sensitive to alcohol. This suggested that in neoplastic cells ABH determinants are not only fewer but also disproportionately represented on polar (alcohol‐soluble) molecules. Alcohol more profoundly affected tissues from nonsecretors probably because the proportion of alcohol‐soluble A, B, H substances differs in secretors and nonsecretors.