Differential regulation of immature articular cartilage compressive moduli and Poisson's ratios by in vitro stimulation with IGF-1 and TGF-β1
Differential regulation of immature articular cartilage compressive moduli and Poisson's ratios by in vitro stimulation with IGF-1 and TGF-β1
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DOI：
10.1016/j.jbiomech.2010.05.022
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发表时间：
2010-09-17
影响因子：
2.4
通讯作者：
Sah, Robert L.
中科院分区：
文献类型：
--
作者：
Williams, Gregory M.;Dills, Kristin J.;Sah, Robert L.
通过研究体内发育和体外培养中胰岛素样生长因子-1(IGF-1)和转化生长因子-β1(TGF-β1)的组成-功能动力学，阐明了关节软骨生长和成熟的机制。这项研究测试了IGF-1和TGF-β1调节未成熟软骨压缩模数和泊松比的假设，其方式与已知的对拉伸性能的影响一致。取S关节浅层和生长中期(M)区的小牛关节软骨，在含有胰岛素样生长因子-1或转化生长因子-β1的培养液中进行新鲜或继之培养，观察其在限制性压缩(CC)和非限制性压缩(UCC)下的力学性能、软骨基质成分和移植块大小。加入IGF-1培养后，CC和UCC软化，泊松比增加，组织体积显著增加，糖胺聚糖(GAG)和胶原(Col)积聚。加入转化生长因子-β1的培养可促进S层的成熟变化，包括CC和UCC的硬化和GAG浓度的增加。COL和吡啶啉交联物(PYR)，但几乎没有增长。含有转化生长因子-β1的M层外植体的培养接近于动态平衡。在所有治疗组中，CC和UCC的压缩模数与GAG、COL和PYR浓度呈正相关，而泊松比与这些基质成分的浓度呈负相关。因此，胰岛素样生长因子-1和转化生长因子-β1在体外对未成熟关节软骨的压缩力学性能和大小具有不同的调节作用。通过使用这些生长因子控制体外生化环境来控制组织生长、成熟或动态平衡，可能在软骨修复和组织工程中有应用。(C)2010爱思唯尔有限公司。保留所有权利。
Mechanisms of articular cartilage growth and maturation have been elucidated by studying composition-function dynamics during in vivo development and in vitro culture with stimuli such as insulin-like growth factor-1 (IGF-1) and transforming growth factor-beta 1 (TGF-beta 1). This study tested the hypothesis that IGF-1 and TGF-beta 1 regulate immature cartilage compressive moduli and Poisson's ratios in a manner consistent with known effects on tensile properties. Bovine calf articular cartilage from superficial-articular (S) and middle-growth (M) regions were analyzed fresh or following culture in medium with IGF-1 or TGF-beta 1. Mechanical properties in confined (CC) and unconfined (UCC) compression, cartilage matrix composition, and explant size were assessed. Culture with IGF-1 resulted in softening in CC and UCC, increased Poisson's ratios, substantially increased tissue volume, and accumulation of glycosaminoglycan (GAG) and collagen (COL). Culture with TGF-beta 1 promoted maturational changes in the S layer, including stiffening in CC and UCC and increased concentrations of GAG. COL and pyridinoline crosslinks (PYR), but little growth. Culture of M layer explants with TGF-beta 1 was nearly homeostatic. Across treatment groups, compressive moduli in CC and UCC were positively related to GAG, COL and PYR concentrations, while Poisson's ratios were negatively related to concentrations of these matrix components. Thus, IGF-1 and TGF-beta 1 differentially regulate the compressive mechanical properties and size of immature articular cartilage in vitro. Prescribing tissue growth, maturation, or homeostasis by controlling the in vitro biochemical environment with such growth factors may have applications in cartilage repair and tissue engineering. (C) 2010 Elsevier Ltd. All rights reserved.