Acupuncture stimulation at Baihui acupoint reduced cerebral infarct and increased dopamine levels in chronic cerebral hypoperfusion and ischemia-reperfusion injured Sprague-Dawley rats
Acupuncture stimulation at Baihui acupoint reduced cerebral infarct and increased dopamine levels in chronic cerebral hypoperfusion and ischemia-reperfusion injured Sprague-Dawley rats
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DOI：
10.1142/s0192415x07005260
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发表时间：
2007-01-01
影响因子：
5.7
通讯作者：
Lin, Jaung-Geng
中科院分区：
文献类型：
--
作者：
Chuang, Chin-Min;Hsieh, Ching-Liang;Lin, Jaung-Geng
百会穴有三阳五聚之功，中医认为针刺百会穴可提神醒脑。因此，本研究旨在探讨针刺百会穴对脑梗死及多巴胺水平的影响。永久性结扎SD大鼠双侧颈总动脉建立慢性脑低灌注动物模型;阻断双侧颈总动脉和右侧大脑中动脉血流90 min后再灌注建立脑梗死动物模型。刺激百会穴20 min，每周3次，共4周。采用8臂放射状迷宫实验评价大鼠的认知和记忆功能，每周1次，连续4周。最后，处死大鼠，取出大脑;测量脑组织中的多巴胺水平，并计算右半球与左半球面积的百分比。结果表明，针刺刺激并不能提高慢性脑低灌注和脑缺血再灌注损伤大鼠模型的八臂径向迷宫的成功率。AS能增加慢性脑低灌注大鼠右侧大脑皮质和海马多巴胺含量，增加脑缺血再灌注损伤大鼠大脑皮质多巴胺含量。再灌注后24小时，对照组和AS组之间的神经功能缺损评分相似，而由缺血再灌注损伤大鼠组成的AS组的右半球至左半球面积的百分比大于对照组。
The Baihui acupoint has three Yang and five convergences; it is needled in order to activate spirit and resuscitate the brain in traditional Chinese medicine. Therefore, the purpose of the present study is to investigate the effect of acupuncture stimulation at the Baihui acupoint on cerebral infarct and dopamine levels. A chronic cerebral hypoperfusion animal model was established by permanent ligation of both common carotid arteries; a cerebral infarct animal model was established by blocking the blood flow of both common carotid arteries and the right middle cerebral artery for 90 min followed by reperfusion in Sprague-Dawly (SD) rats. The Baihui acupoint was stimulated for 20 min 3 days per week for 4 weeks. The cognitive and memory functions were evaluated by measuring the successful rates for rats to negotiate an 8-arm radial maze test; the test was performed after operation once a week for 4 weeks. Finally, the rats were sacrificed and their brains were removed; the dopamine levels in brain tissue were measured and the percentage of right to left hemisphere area was calculated. The results indicated that acupuncture stimulation ( AS) did not increase the success rate of performing the 8-arm radial maze in chronic cerebral hypoperfusion and cerebral ischemia-reperfusion injured rat models. AS increased dopamine levels in the right cerebral cortex and hippocampus in the chronic cerebral hypoperfusion rats, and increased dopamine levels of the cerebral cortex in the cerebral ischemia-reperfusion injured rat's models. The neurological deficit score was similar between control and AS groups 24 hours after reperfusion, whereas the AS group comprised of ischemia-reperfusion injured rats had a greater percentage of right to left hemisphere area than the control group.