DIARRHEA DUE TO ESCHERICHIA-COLI IN RABBIT - NOVEL MECHANISM
DIARRHEA DUE TO ESCHERICHIA-COLI IN RABBIT - NOVEL MECHANISM
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DOI：
10.1093/infdis/135.3.454
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发表时间：
1977-01-01
影响因子：
6.4
通讯作者：
BLAKE, RK
中科院分区：
文献类型：
--
作者：
CANTEY, JR;BLAKE, RK
大肠杆菌菌株对从几只腹泻家兔中分离的大肠杆菌O 15（RDEC-1）进行了检测，以确定该菌株经口胃途径给药于其他家兔时是否会引起腹泻，以及该菌株是否具有侵袭性或肠致病性。当通过口胃途径以1.5 × 10 - 6的剂量给予时，菌株RDEC-1在62只兔子中的48只中产生腹泻。102至4倍。1010个细菌。该微生物在18小时回肠袢或Sereny试验中未给出阳性结果，并且未侵入[人宫颈癌] HeLa细胞。在6或18小时兔回肠袢、乳鼠、[小鼠] Y-1肾上腺细胞或中国仓鼠卵巢细胞试验中，菌株RDEC-1的培养物上清液未得到阳性结果。用荧光抗体染色法检测肠组织切片中的大肠杆菌。大肠杆菌RDEC-1菌株粘附于上皮表面。E.大肠杆菌显然可以在不侵入粘膜或合成肠毒素的情况下引起腹泻。株大肠与RDEC-1相似的大肠杆菌可能解释了部分E.与大肠杆菌有关的人类腹泻。
A strain of E. coli O15 (RDEC-1) isolated from several rabbits with diarrhea was examined to determine whether the strain could produce diarrhea when administered by the orogastric route to other rabbits and whether this strain was invasive or enterotoxigenic. Strain RDEC-1 produced diarrhea in 48 of 62 rabbits when given by the orogastric route in doses that ranged from 1.5 .times. 102 to 4 .times. 1010 bacteria. The organism did not give a positive result in the 18 h ileal loop or Sereny tests and did not invade [human cervical carcinoma] HeLa cells. Culture supernatants of strain RDEC-1 did not give positive results in the 6- or 18-h rabbit ileal loop, suckling mouse, [mouse] Y-1 adrenal cell or Chinese hamster ovary cell assays. Fluorescent antibody staining of sections of intestine prepared in a cryostat revealed great numbers of E. coli strain RDEC-1 that adhered to the epithelial surface. E. coli apparently can produce diarrhea without being able to invade the mucosa or synthesize enterotoxin. Strains of E. coli similar to RDEC-1 may account for some of the E. coli-associated diarrhea that occurs in humans.