THE HALF-LIFE OF ENDOGENOUS INSULIN AND C-PEPTIDE IN MAN ASSESSED BY SOMATOSTATIN SUPPRESSION
THE HALF-LIFE OF ENDOGENOUS INSULIN AND C-PEPTIDE IN MAN ASSESSED BY SOMATOSTATIN SUPPRESSION
复制标题
DOI：
10.1111/j.1365-2265.1985.tb00185.x
复制
发表时间：
1985-01-01
影响因子：
3.2
通讯作者：
TURNER, RC
中科院分区：
文献类型：
--
作者：
MATTHEWS, DR;RUDENSKI, AS;TURNER, RC
在正常人中，通过检查生长抑素抑制分泌后内源性产生的胰岛素和C肽的下降的方法，评估胰岛素和C肽的体内半衰期。每分钟从7名正常受试者中采集静脉血样：在1分钟内静脉注射葡萄糖（0.1g/kg理想体重）以刺激分泌，随后在随后的30分钟内推注250 μ g生长抑素-14和再输注250 μ g生长抑素-14。分析血浆样品的C-肽、葡萄糖和胰岛素。在8分钟内的初始单指数半衰期为3.9 ±。0.3和10.2 .+-。0.7 min（平均值±. SEM [平均值的标准误差]），随后下降较慢。对数转换的胰岛素和C肽产生双相下降，通过2池模型进行评估。胰岛素清除的速率常数暗示了主动摄取，而C肽清除的动力学较慢，不可逆的损失可能仅由肾小球滤过解释。测量激素动力学的生长抑素抑制方法可用于新描述的激素，这些激素不可用于体内研究。
The in-vivo half-lives of insulin and C-peptide were assessed in normal man by a method which examines the decline of endogenously produced insulin and C-peptide after somatostatin suppression of secretion. Venous blood samples were taken each minute from 7 normal subjects: i.v. glucose (0.1 g/kg ideal body wt) was given over 1 min to stimulate secretion, followed by a bolus of 250 .mu.g of somatostatin-14 and an infusion of a further 250 .mu.g somatostatin-14 over the subsequent 30 min. Plasma samples were analyzed for C-peptide, glucose and insulin. The initial mono-exponential half-lives over 8 min were 3.9 .+-. 0.3 and 10.2 .+-. 0.7 min respectively (mean .+-. SEM [standard error of the mean]) with subsequent slower declines. Log transformed insulin and C-peptide yielded biphasic declinations which were assessed by a 2-pool model. The rate constant of clearance of insulin implied avid uptake, while the kinetics of C-peptide clearance were slower and irreversible loss might be explained by glomerular filtration alone. The somatostatin suppression method of measuring hormone kinetics could be used for newly described hormones which are not available for in-vivo studies.