Enhancement of lipid accumulation and docosahexaenoic acid synthesis in Schizochytrium sp. H016 by exogenous supplementation of sesamol
Enhancement of lipid accumulation and docosahexaenoic acid synthesis in Schizochytrium sp. H016 by exogenous supplementation of sesamol
复制标题
裂殖壶菌中脂质积累和二十二碳六烯酸合成的增强。
DOI：
10.1016/j.biortech.2021.126527
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发表时间：
2021-12-13
影响因子：
11.4
通讯作者：
Yu, Longjiang
中科院分区：
文献类型：
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作者：
Bao, Zhendong;Zhu, Yuanmin;Yu, Longjiang
裂殖酵母属(Schizochytrium sp.)是工业化生产二十二碳六烯酸(DHA)最有前景的海洋产油微生物之一。在本研究中，外源添加1 mM芝麻酚可有效地阻止发酵过程中多不饱和脂肪酸的过氧化，从而显著提高油脂产量和DHA产量，分别提高53.52%和78.30%。芝麻酚的加入还提高了细胞的总抗氧化能力，并诱导了聚酮合成酶和抗氧化酶系统的基因表达。此外，芝麻酚通过抑制苹果酸酶活性和促进葡萄糖-6-磷酸脱氢酶活性来调节烟酰胺腺嘌呤二核苷酸磷酸的供应。补料分批发酵结果表明，添加芝麻酚可显著提高DHA产量90.76%。本研究为提高裂殖酵母DHA产量提供了重要参考。在工业发酵中。
Schizochytrium sp. is one of the most promising marine oleaginous microorganisms for industrial production of docosahexaenoic acid (DHA). In this study, the exogenous supplementation of 1 mM sesamol to the fermentation medium effectively prevented the peroxidation of polyunsaturated fatty acids in the fermentation process, which thereby significantly increasing the lipid and DHA yield by 53.52% and 78.30%, respectively. The addition of sesamol also increased the total antioxidant capacity of cells and induce the gene expression of polyketide synthase and antioxidant enzyme system. Moreover, the supply of nicotinamide adenine dinucleotide phosphate was regulated by sesamol by inhibiting the malic enzyme activity and promoting the glucose-6-phosphate dehydrogenase activity. Finally, fed-batch fermentation showed that the addition of sesamol significantly enhanced the DHA yield by 90.76%. This study provides an important reference for enhancing the DHA productivity of Schizochytrium sp. in industrial fermentation.