Dugbe nairovirus S segment: Correction of published sequence and comparison of five isolates
Dugbe nairovirus S segment: Correction of published sequence and comparison of five isolates
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DOI：
10.1006/viro.2001.1324
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发表时间：
2002-03-15
期刊：
影响因子：
3.7
通讯作者：
Elliott, RM
中科院分区：
文献类型：
--
作者：
Bridgen, A;Dalrymple, DA;Elliott, RM
Dugbe内罗病毒（DUG）的ArD 44313分离物的S（小）RNA片段的序列已被重新测定，并且在先前报道的序列中存在许多明显的错误（V K Ward，A. C.马里奥特A、El-Ghorr和RA. Nuttall，1990，Virology 175，518-524）。我们的结果表明S RNA长度为1716个核苷酸（nt），包含一个跨越1449个nt的大开放阅读框。这可以编码483个氨基酸的多肽M，53.9kDa，对应于病毒核衣壳蛋白N。因此，DUG N蛋白的长度与哈扎拉（HAZ）和克里米亚-刚果出血热（CCHF）内罗病毒的N蛋白相似，其长度分别为485和482个氨基酸。还测定了DUG分离物IbAr 1792、IbH 11480、ArD 16095和KT 281/75的S节段RNA序列;只有KT 281/76序列与ArD 44313的序列显著不同。构建了这些内罗病毒S片段序列的系统发育树。(C)2002 Elsevier Science（美国）。
The sequence of the S (small) RNA segment of the ArD 44313 isolate of Dugbe nairovirus (DUG) has been redetermined, and a number of apparent errors in the previously reported sequence (V K Ward, A. C. Marriott, A. A, El-Ghorr, and R A. Nuttall, 1990, Virology 175, 518-524) were revealed. Our results indicate that the S RNA is 1716 nucleotides (nt) in length and contains one large open reading frame spanning 1449 nt. This can encode a 483 amino acid polypeptide, M, 53.9 kDa, corresponding to the viral nucleocapsid protein N. The DUG N protein is thus similar in length to the N proteins of Hazara (HAZ) and Crimean-Congo haemorrhagic fever (CCHF) nairoviruses, which are 485 and 482 amino acids in length, respectively, S segment RNA sequences were also determined for DUG isolates IbAr 1792, IbH 11480, ArD 16095, and KT 281/75; only the KT 281/76 sequence differed markedly from that of ArD 44313. Phylogenetic trees were constructed for these nairovirus S segment sequences. (C) 2002 Elsevier Science (USA).