Menstrual cycle-dependent changes of Toll-like receptors in endometrium
Menstrual cycle-dependent changes of Toll-like receptors in endometrium
复制标题
DOI：
10.1093/humrep/del388
复制
发表时间：
2007-02-01
影响因子：
6.1
通讯作者：
Fazeli, A.
中科院分区：
文献类型：
--
作者：
Aflatoonian, R.;Tuckerman, E.;Fazeli, A.
背景：对感染的快速先天免疫防御通常涉及通过特定的模式识别受体识别入侵的病原体，最近归因于toll样受体家族（TLRs）。我们实验室和其他实验室的报告已经证明了TLRs 1-6在女性生殖道中存在。然而，鉴别TLRs 7-10在女性生殖道，特别是子宫中的作用却很少。此外，关于TLRs在月经周期中在女性生殖道中的变化的信息也很少。方法：采用免疫染色法检测正常女性子宫内膜定时活检组织中TLR7-10蛋白的分布。采用RT-PCR方法证实了TLR1-10基因在子宫内膜组织中的存在，并采用real-time PCR方法分析了这些基因在正常人子宫内膜月经周期中的相对表达情况。结果：子宫内膜上皮及间质中检测到TLR7-10蛋白。TLR1-10基因在人子宫内膜组织中表达，TLR2-6、9和10基因在分泌期的平均相对表达量明显高于月经周期的其他阶段。结论：TLR7-10定位不仅局限于子宫内膜上皮，也存在于子宫内膜组织间质中。子宫内膜tlr2 - 6,9和10基因在月经周期中周期性表达。
BACKGROUND: Rapid innate immune defences against infection usually involve the recognition of invading pathogens by specific pattern recognition receptors recently attributed to the family of Toll-like receptors (TLRs). Reports from our laboratory and others have demonstrated the existence of TLRs 1-6 in the female reproductive tract. However, little has been done to identify TLRs 7-10 in the female reproductive tract, particularly in the uterus. Also little information exists regarding variation in TLRs in the female reproductive tract during the menstrual cycle. METHOD: The distribution of TLR7-10 protein was detected by immunostaining in timed endometrial biopsies from normal women. RT-PCR was used to show the existence of TLR1-10 genes in endometrial tissue and real-time PCR analysis to investigate the relative expression of these genes during the menstrual cycle in normal human endometrium. RESULTS: TLR7-10 proteins were detected in endometrial epithelium and stroma. TLR1-10 genes were expressed in human endometrial tissue, and the mean relative expression of TLR2-6, 9 and 10 genes was significantly higher during the secretory phase compared with other phases of the menstrual cycle. CONCLUSIONS: TLR7-10 localization is not limited to endometrial epithelium but is also present in the stroma of the endometrial tissue. Endometrial TLR2-6, 9 and 10 genes are cyclically expressed during the menstrual cycle.