TRIM14 Promotes Breast Cancer Cell Proliferation by Inhibiting Apoptosis.
TRIM14 Promotes Breast Cancer Cell Proliferation by Inhibiting Apoptosis.
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DOI：
10.3727/096504018x15214994641786
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发表时间：
2019-03-29
影响因子：
3.1
通讯作者：
Wang M
中科院分区：
文献类型：
--
作者：
Hu G;Pen W;Wang M
TRIM 14在几种人类癌症中异常表达。然而，TRIM 14在人类乳腺癌中的功能和表达仍然在很大程度上未知。为了了解TRIM 14在乳腺癌中的生物学功能，我们测量了TRIM 14的表达水平。在TRIM 14过表达或敲低后测量细胞增殖和细胞凋亡。在人乳腺癌标本和细胞系中发现了TRIM 14的上调。在BT474和MDA-MB-231细胞系中，TRIM 14的减少抑制细胞增殖，但增加细胞凋亡。进一步的研究表明，TRIM 14的敲低上调了BAX的表达，同时下调了BCL 2的表达。此外，SHP-1的表达增加，STAT 3的磷酸化（p-STAT 3）被抑制。相反，TRIM 14的过表达具有相反的效果。此外，隐丹参酮，STAT 3抑制剂，抑制细胞增殖，但增加细胞凋亡的BT474和MDA-MB-231细胞系。结论：TRIM 14可能作为一个癌基因在人类乳腺癌中发挥作用，并可能成为人类乳腺癌的一种新策略。
Tripartite motif-containing 14 (TRIM14) is abnormally expressed in several human cancers. However, the function and expression of TRIM14 in human breast cancer are still largely unknown. To understand the biological function of TRIM14 in breast cancer, we measured the expression level of TRIM14. Cell proliferation and cell apoptosis were measured after TRIM14 overexpression or knockdown. Upregulation of TRIM14 was found in human breast cancer specimens and cell lines. Reduction of TRIM14 inhibited cell proliferation but increased cell apoptosis in the BT474 and MDA-MB-231 cell lines. Further study showed that knockdown of TRIM14 upregulated the expression of BAX while downregulating the expression of BCL2. In addition, the expression of SHP-1 was increased, and the phosphorylation of STAT3 (p-STAT3) was inhibited. Conversely, overexpression of TRIM14 had the opposite effects. Additionally, cryptotanshinone, a STAT3 inhibitor, inhibited cell proliferation but increased cell apoptosis in the BT474 and MDA-MB-231 cell lines. In conclusion, TRIM14 may act as an oncogene in human breast cancer and may be a novel strategy for human breast cancer.