Enhanced Antibacterial Activity of Hen Egg-White Lysozyme against Staphylococcus aureus and Escherichia coli due to Protein Fibrillation
Enhanced Antibacterial Activity of Hen Egg-White Lysozyme against Staphylococcus aureus and Escherichia coli due to Protein Fibrillation
复制标题
鸡蛋清溶菌酶因蛋白纤维化而增强对金黄色葡萄球菌和大肠杆菌的抗菌活性
DOI：
10.1021/acs.biomac.0c01599
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发表时间：
2021-01-19
影响因子：
6.2
通讯作者：
Xiang, Ning
中科院分区：
文献类型：
--
作者：
Wei, Zhengxun;Wu, Sihong;Xiang, Ning
具有广谱抗菌性能的抗菌剂一直需求量很大。溶菌酶是一种常见而廉价的蛋白质，广泛应用于食品安全和生物医学领域的抗菌应用。然而，许多病原体对溶菌酶具有抗性或不敏感。在本研究中，我们研究了蛋清溶菌酶形成的寡聚体和淀粉样原纤维(HEWL)对金黄色葡萄球菌和大肠杆菌的抑菌活性及其机制。HEWL纤维对溶菌酶耐药的金黄色葡萄球菌和溶菌酶不敏感的大肠杆菌均表现出显著增强的抗菌活性。另一方面，与天然的HEWL相比，HEWL寡聚体的抗菌活性没有明显的提高。我们的结果表明，HEWL的原纤化可以显著增强对金黄色葡萄球菌和大肠杆菌的抗菌活性。天然廉价的Hewl类淀粉原纤维可潜在地应用于抗菌食品包装、动物饲料、抗生素替代等领域。
Antibacterial agents with broad-spectrum antibacterial properties have always been in large demand. Lysozyme, a common and inexpensive protein, is widely used in food safety and biomedical applications for antibacterial purposes. However, many pathogens are lysozyme-resistant or insensitive. In this research, we investigated the antibacterial activities and mechanism of oligomers and amyloid fibrils formed from hen egg-white lysozyme (HEWL) against Staphylococcus aureus and Escherichia coli. The HEWL fibrils showed significantly enhanced antibacterial activity against both lysozyme-resistant S. aureus and lysozyme-insensitive E. coli. The HEWL oligomers, on the other hand, did not show an obvious improvement in antibacterial activity compared to native HEWL. Our results indicated that the fibrillation of HEWL can significantly enhance antibacterial activity against both S. aureus and E. coli. The natural and inexpensive HEWL amyloid fibrils can be potentially applied to antimicrobial food packaging, animal feed, antibiotic replacement, etc.