Cell type-specific control of human neuronectin secretion by polypeptide mediators and phorbol ester.
Cell type-specific control of human neuronectin secretion by polypeptide mediators and phorbol ester.
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DOI：
10.1177/37.12.2685108
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发表时间：
1989-12
期刊：
影响因子：
--
通讯作者：
W. Rettig;P. Garin‐Chesa
中科院分区：
文献类型：
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作者：
W. Rettig;P. Garin‐Chesa
神经连接素（Neuronectin，NEC 1）是一种人细胞外基质（extracellular matrix，ECM）蛋白，在正常成人中枢神经系统的白色物质中以独特的吻尾模式表达。此外，NEC 1在正常胎儿和成人平滑肌中表达，沿着某些上皮-间充质连接，并在发育中的胎儿软骨中短暂表达。在皮肤伤口和光化性角化病、银屑病皮肤病变和一系列恶性肿瘤的反应性基质中发现了区域特异性NEC 1诱导。对这些不同组织模式的一种解释是，能够产生NEC 1的细胞广泛分布在神经和间充质组织中，但它们只有在区域特异性分化信号诱导下才能成为NEC 1生产者。在这项研究中，我们使用培养的人类细胞来显示几种调节多肽，包括成纤维细胞生长因子、肿瘤坏死因子、血小板衍生生长因子、神经生长因子和转化生长因子-β，（TGF-β）以及12-O-十四烷酰基佛波醇-13-乙酸酯（TPA）调节NEC 1分泌，在不同的神经和间充质细胞类型中具有不同的诱导和抑制活性模式。TPA和TGF-β作为NEC 1分泌的诱导剂和抑制剂，这取决于靶细胞。这些作用是特异性的NEC 1和没有看到其他几个分泌和膜蛋白的研究。我们认为，NEC 1的表达在不同的细胞系和组织损伤和肿瘤的反应中受到不同的外源性控制。
Neuronectin (NEC1) is a human extracellular matrix (ECM) protein expressed with a unique rostrocaudal pattern in white matter of the normal adult central nervous system. In addition, NEC1 is expressed in normal fetal and adult smooth muscle, along certain epithelial-mesenchymal junctions, and transiently in developing fetal cartilage. Region-specific induction of NEC1 is found in dermal wounds and in the reactive stroma of actinic keratoses, psoriatic skin lesions, and a range of malignant tumors. One explanation for these diverse tissue patterns is that cells capable of producing NEC1 are widely distributed in neural and mesenchymal tissues, but they become NEC1 producers only when induced by region-specific differentiation signals. In this study, we used cultured human cells to show that several regulatory polypeptides, including fibroblast growth factors, tumor necrosis factor, platelet-derived growth factor, nerve growth factor, and transforming growth factor-beta (TGF-beta), as well as 12-O-tetradecanoyl phorbol-13-acetate (TPA), modulate NEC1 secretion, with distinct patterns of inducing and inhibitory activities in different neural and mesenchymal cell types. TPA and TGF-beta act both as inducers and inhibitors of NEC1 secretion, depending on the target cell. These effects are specific for NEC1 and are not seen for several other secreted and membrane proteins studied. We suggest that NEC1 expression comes under different modes of extrinsic control in different cell lineages and in response to tissue injury and neoplasia.