Tracking the rejection and survival of mouse ovarian iso- and allografts in vivo with bioluminescent imaging
Tracking the rejection and survival of mouse ovarian iso- and allografts in vivo with bioluminescent imaging
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DOI：
10.1530/rep-09-0448
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发表时间：
2010-07-01
期刊：
影响因子：
3.8
通讯作者：
Tzeng, Chii-Ruey
中科院分区：
文献类型：
--
作者：
Chen, Chi-Huang;Yeh, Yu-Chi;Tzeng, Chii-Ruey
荧光素酶报告基因的体内生物发光成像技术在生物医学领域有着广泛的应用。荧光素酶转基因小鼠是追踪移植卵巢组织的非常有用的供体。实现该系统的全部潜力可能极大地有利于研究移植移植物的生理行为和功能，以及快速和可靠地评估新的移植方案。以荧光素酶转基因为报告基因，将供体FVB/N-Tg（PolII-Luc）Ltc转基因小鼠的卵巢组织移植到同种/异基因受体中。随着时间的推移，在体内定量分析排斥反应、卵巢功能和BLI。卵巢同种移植物的BLI显示比同种异体移植物的生存期更长，即使用环孢素A（CsA）治疗。排斥反应时，同种异体移植物外周血T淋巴细胞的CD 4（+）/CD 8（+）C比值明显低于同系移植物（P < 0.0001），而CD 19（+）细胞则高于同系移植物。从第1天起，同种异体移植物中的CD 4（+）/CD 8（+）C细胞浸润并不明显，但从第8天起，同种异体移植物中的CD 4（+）/CD 8（+）C细胞浸润很强。激素活性显示完全发情周期的同系移植物，但只有间情期的同种异体移植物。这些结果表明，BLI在体内加速卵巢移植物的快速通量和命运图。使用BLI纵向监测卵巢移植物的免疫排斥反应表明，同种异体移植物的存活时间短，而同种异体移植物的存活时间长得多。CsA单独治疗对卵巢移植的急性排斥反应无效。生殖（2010年）140 105-112
The applications of in vivo bioluminescent imaging (BLI) with a luciferase reporter gene occur widely across biomedical fields. Luciferase-transgenic mice are highly useful donors for tracking transplanted ovarian tissues. Realizing the full potential of this system may greatly benefit the study of the physiological behaviour and function of transplanted grafts, and the rapid and reliable evaluation of new transplantation protocols. The ovarian tissues of donor FVB/N-Tg(PolII-Luc)Ltc transgenic mice, with a luciferase transgene as the reporter, were transplanted into iso/allogeneic recipients. Rejection, ovarian function and BLI were quantitatively analysed in vivo over time. The BLI of the ovarian isografts revealed longer survival than that of allografts, even with cyclosporineA(CsA) treatment. TheCD4(+)/CD8(+) C ratios of peripheral T-cells were significantly reduced in allografts compared with those in isografts (P < 0.0001) during rejection, whereas CD19(+) cell numbers were higher in allografts. The infiltration of CD4(+)/CD8(+) C cells into the graft was unremarkable in isografts from day 1, but was strong in allografts from day 8 onwards. Hormone activity revealed complete oestrus cycles in the isografts but only the dioestrus stage in the allografts. These results demonstrate that BLI in vivo expedites the fast throughput and fate maps of ovarian grafts. The use of BLI to longitudinally monitor ovarian grafts for immunorejection demonstrated the short survival of allografts and the much longer survival of isografts. CsA treatment alone is ineffective against the acute rejection of ovarian allografts. Reproduction (2010) 140 105-112