Occurrence of immunoreactivity for adipocyte-type fatty acid binding protein in degenerating granulosa cells in atretic antral follicles of mouse ovary
Occurrence of immunoreactivity for adipocyte-type fatty acid binding protein in degenerating granulosa cells in atretic antral follicles of mouse ovary
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DOI：
10.1007/s10735-006-9024-y
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发表时间：
2005-10-01
影响因子：
3.2
通讯作者：
Kondo, Hisatake
中科院分区：
文献类型：
--
作者：
Nourani, Mohammad Reza;Owada, Yuji;Kondo, Hisatake
通过免疫光和电子显微镜检查成熟小鼠卵巢中脂肪细胞型脂肪酸结合蛋白（A-FABP）的定位。在 1-6 个窦卵泡中检测到对 A-FABP 具有独特免疫反应性的孤立圆形细胞。在分别针对A-FABP和TUNEL反应的免疫反应性进行处理的载玻片上镜面对准的组中，对A-FABP具有免疫反应性的细胞出现在与含有表现出TUNEL反应的细胞相同的窦卵泡中。在免疫电子显微镜中，发现 A-FABP 免疫阳性细胞含有圆形、不规则或新月形的高电子密度细胞核以及细胞质残余物，没有任何巨噬细胞或外源细胞的特征。因此，该细胞被鉴定为凋亡的颗粒细胞。对 A-FABP 具有免疫反应性的凋亡细胞经常被观察到被包围/吞噬在表现出正常超微结构且不含大量溶酶体的相邻细胞中。目前的研究结果表明，A-FABP 可能通过其与过氧化物酶体增殖物激活受体的相互作用参与卵巢颗粒细胞的凋亡。
The localization of adipocyte-type fatty acid binding protein (A-FABP) in the mature mouse ovary was examined by immuno-light and electron microscopy. Solitary round cells showing the distinct immunoreactivity for A-FABP were detected in 1-6 antral follicles. In sets of two consecutive sections in a mirror alignment on slide glasses which were treated for immunoreactivity for A-FABP and TUNEL reaction separately, cells immunoreactive for A-FABP appeared in the same antral follicles as containing cells exhibiting TUNEL-reaction. In immunoelectron microscopy, A-FABP-immunopositive cells were found to contain highly electron-dense nuclei of round, irregular or crescent shapes together with cytoplasmic remnants without any features of macrophages or cells of extrinsic origin. Therefore the cells were identified as apoptotic granulosa cells. The apoptotic cells immunoreactive for A-FABP were often seen to be enclosed/engulfed in adjacent cells exhibiting normal ultrastructures without containing numerous lysosomes. The present findings suggest that A-FABP is involved in the apoptosis of ovarian granulosa cells, probably through its interaction with peroxisome proliferator activated receptors.