A PATTERN OF ACCUMULATION OF A SOMATIC DELETION OF MITOCHONDRIAL-DNA IN AGING HUMAN TISSUES
A PATTERN OF ACCUMULATION OF A SOMATIC DELETION OF MITOCHONDRIAL-DNA IN AGING HUMAN TISSUES
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DOI：
10.1073/pnas.89.16.7370
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发表时间：
1992-08-15
影响因子：
11.1
通讯作者：
ARNHEIM, N
中科院分区：
文献类型：
--
作者：
CORTOPASSI, GA;SHIBATA, D;ARNHEIM, N
一种选择性地放大线粒体基因组的特定缺失的分析已经被用来研究这种缺失在各种人类组织中积累的程度。这种缺失在神经和肌肉组织中的水平比在所有其他研究的组织中都要高得多。在年龄相近的不同人中，相同组织之间的缺失水平的差异似乎小于个体内组织之间的差异。对水平差异的人为解释的测试都是否定的。确定了与缺失程度相关的三个细胞参数。有害的线粒体突变在有限的老化人类组织亚群中优先积累，可能会加剧这些组织中随着年龄增长而增加的功能缺陷。
An assay that selectively amplifies a specific deletion of the mitochondrial genome has been used to study the extent of the deletion's accumulation in a variety of human tissues. The deletion occurs at much higher levels in nervous and muscle tissues than in all other tissues studied. The variation in deletion level between the same tissues in different persons of similar age appears to be less than the variation among tissues within an individual. Tests for artifactual explanations of the level differences were each negative. Three cellular parameters that are correlated with the level of the deletion are identified. The preferential accumulation of deleterious mitochondrial mutations in a restricted subset of aging human tissues may compound deficiencies of function in those tissues that accrue with age.