In Vitro Models of Blood and Lymphatic Vessels-Connecting Tissues and Immunity.
In Vitro Models of Blood and Lymphatic Vessels-Connecting Tissues and Immunity.
复制标题
连接组织和免疫的血液和淋巴管的体外模型。
DOI：
10.1002/adbi.202200041
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发表时间：
2023
期刊：
影响因子：
3.7
通讯作者：
Maisel,Katharina
中科院分区：
文献类型：
--
作者：
Bogseth,Amanda;Ramirez,Ann;Vaughan,Erik;Maisel,Katharina
血管和淋巴管是生理过程的调节器，包括氧合和液体运输。这两条血管遍布全身，对于维持组织稳态至关重要。每艘船舶流程的复杂性限制了人们对船舶到底如何维持其功能的理解。这两条血管已被证明参与许多疾病的发病机制，包括癌症转移，进一步探讨所涉及的具体机制至关重要。开发体外模型是为了更好地了解血液和淋巴的生理功能及其机制。在这篇综述中，描述了血液和淋巴体外模型系统，包括使用 Transwell、微流体装置、类器官培养和各种其他方法进行的 2D 和 3D 设计。研究内皮细胞-细胞外基质相互作用、内皮屏障特性、跨内皮转运和细胞迁移、淋巴/血管生成、血管炎症和内皮-癌细胞相互作用的模型受到特别关注。虽然该领域在淋巴管和血管系统的建模和理解方面取得了重大进展，但更多的模型，包括多种细胞类型的共培养、复杂的细胞外基质和 3D 形态，特别是模拟疾病状态的模型，将有助于进一步了解血液和淋巴管系统在健康和疾病中的作用。
Blood and lymphatic vessels are regulators of physiological processes, including oxygenation and fluid transport. Both vessels are ubiquitous throughout the body and are critical for sustaining tissue homeostasis. The complexity of each vessel's processes has limited the understanding of exactly how the vessels maintain their functions. Both vessels have been shown to be involved in the pathogenesis of many diseases, including cancer metastasis, and it is crucial to probe further specific mechanisms involved. In vitro models are developed to better understand blood and lymphatic physiological functions and their mechanisms. In this review, blood and lymphatic in vitro model systems, including 2D and 3D designs made using Transwells, microfluidic devices, organoid cultures, and various other methods, are described. Models studying endothelial cell‐extracellular matrix interactions, endothelial barrier properties, transendothelial transport and cell migration, lymph/angiogenesis, vascular inflammation, and endothelial‐cancer cell interactions are particularly focused. While the field has made significant progress in modeling and understanding lymphatic and blood vasculature, more models that include coculture of multiple cell types, complex extracellular matrix, and 3D morphologies, particularly for models mimicking disease states, will help further the understanding of the role of blood and lymphatic vasculature in health and disease.