SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.
SCF E3 ligase PP2-B11 plays a positive role in response to salt stress in Arabidopsis.
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SCF E3 连接酶 PP2-B11 在拟南芥应对盐胁迫中发挥积极作用。
DOI：
10.1093/jxb/erv245
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发表时间：
2015-08
影响因子：
6.9
通讯作者：
Zheng C
中科院分区：
文献类型：
--
作者：
Jia F;Wang C;Huang J;Yang G;Wu C;Zheng C
AtPP2-B11是一种F-box蛋白，它通过调节Annat1的表达、抑制活性氧的产生和破坏Na+的稳态来增强拟南芥的耐盐性。Skp1-Cullin-F-box(SCF)E3连接酶对细胞信号转导中许多重要因子的翻译后调控是必不可少的。在本研究中，我们从拟南芥中鉴定出一种F-box蛋白，AtPP2-B11，该蛋白在转录水平和蛋白水平上都随着盐处理时间的延长而显著诱导。高表达AtPP2-B11的转基因拟南芥植株表现出明显的耐盐性，而RNA干扰系比野生型植株对盐胁迫更敏感。相对和绝对定量分析表明，4311个差异表达蛋白受盐胁迫下AtPP2-B11的调控。AtPP2-B11可以上调Annat1基因的表达，在盐胁迫与拟南芥体内活性氧积累之间起着分子纽带的作用。此外，AtPP2-B11还影响了盐胁迫下Na+动态平衡基因的表达，高表达品系表现出较低的Na+积累量。这些结果表明，AtPP2-B11在拟南芥对盐胁迫的响应中起着积极的调节作用。
AtPP2-B11, an F-box protein, enhances the salt stress tolerance by regulating AnnAt1 expression, repressing reactive oxygen species production, and disrupting Na+ homeostasis in Arabidopsis. Skp1–Cullin–F-box (SCF) E3 ligases are essential to the post-translational regulation of many important factors involved in cellular signal transduction. In this study, we identified an F-box protein from Arabidopsis thaliana, AtPP2-B11, which was remarkably induced with increased duration of salt treatment in terms of both transcript and protein levels. Transgenic Arabidopsis plants overexpressing AtPP2-B11 exhibited obvious tolerance to high salinity, whereas the RNA interference line was more sensitive to salt stress than wild-type plants. Isobaric tag for relative and absolute quantification analysis revealed that 4311 differentially expressed proteins were regulated by AtPP2-B11 under salt stress. AtPP2-B11 could upregulate the expression of annexin1 (AnnAt1) and function as a molecular link between salt stress and reactive oxygen species accumulation in Arabidopsis. Moreover, AtPP2-B11 influenced the expression of Na+ homeostasis genes under salt stress, and the AtPP2-B11 overexpressing lines exhibited lower Na+ accumulation. These results suggest that AtPP2-B11 functions as a positive regulator in response to salt stress in Arabidopsis.