Evaluation of Clonostachys rosea for Control of Plant- Parasitic Nematodes in Soil and in Roots of Carrot and Wheat
Evaluation of Clonostachys rosea for Control of Plant- Parasitic Nematodes in Soil and in Roots of Carrot and Wheat
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DOI：
10.1094/phyto-03-17-0091-r
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发表时间：
2018-01-01
期刊：
影响因子：
3.2
通讯作者：
Karlsson, Magnus
中科院分区：
文献类型：
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作者：
Iqbal, Mudassir;Dubey, Mukesh;Karlsson, Magnus
生物防治是减少植物线虫病害的一条很有前途的途径。通过盆栽试验，研究了接种华支睾吸虫IK726菌株对自然线虫侵染土壤中线虫群落组成的影响，以及对植物健康的影响。喷施玫瑰花后，从土壤和植物根部提取的植物寄生线虫属数减少了40%~73%，而非寄生线虫属未受影响。土壤接种玫瑰红可使小麦植株鲜重增加20%，地上部长度增加24%，而胡萝卜仅增加地上部干重48%。通过光学显微镜观察玫瑰花纹夜蛾与线虫的体外相互作用，没有发现直接寄生的证据。而在马铃薯葡萄糖发酵液、麦芽浸膏发酵液和人工营养发酵液中培养滤液对线虫均有一定的毒杀作用，48h内对线虫的固定率分别为57%、62%和100%，说明玫瑰花菇能有效控制植物寄生线虫，从而促进植物生长。这种拮抗作用最可能的机制是通过产生杀线虫化合物来产生抗生素，而不是直接寄生。
Biological control is a promising approach to reduce plant diseases caused by nematodes. We tested the effect of the fungus Clonostachys rosea strain IK726 inoculation on nematode community composition in a naturally nematode infested soil in a pot experiment, and the effect of C. rosea on plant health. The numbers of plant- parasitic nematode genera extracted from soil and plant roots decreased by 40 to 73% when C. rosea was applied, while genera of nonparasitic nematodes were not affected. Soil inoculation of C. rosea increased fresh shoot weight and shoot length of wheat plants by 20 and 24%, respectively, while only shoot dry weight increased by 48% in carrots. Light microscopy of in vitro C. rosea-nematode interactions did not reveal evidence of direct parasitism. However, culture filtrates of C. rosea growing in potato dextrose broth, malt extract broth and synthetic nutrient broth exhibited toxicity toward nematodes and immobilized 57, 62, and 100% of the nematodes, respectively, within 48 h. This study demonstrates that C. rosea can control plant- parasitic nematodes and thereby improve plant growth. The most likely mechanism responsible for the antagonism is antibiosis through production of nematicidal compounds, rather than direct parasitism.