Purification, Characterization, and Application for Preparation of Antioxidant Peptides of Extracellular Protease from Pseudoalteromonas sp. H2
Purification, Characterization, and Application for Preparation of Antioxidant Peptides of Extracellular Protease from Pseudoalteromonas sp. H2
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DOI：
10.3390/molecules24183373
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发表时间：
2019-09-01
期刊：
影响因子：
4.6
通讯作者：
Chen, Leilei
中科院分区：
文献类型：
--
作者：
Liu, Dan;Huang, Jiafeng;Chen, Leilei
该研究报告了从假交替单胞菌中分离出一种名为 EH2 的金属蛋白酶。 H2。 EH2 在 5-10 的广泛 pH 范围内保持超过 80% 的活性，并且稳定性也几乎与 pH 无关。在 20-70 摄氏度的宽温度范围内检测到超过 65% 的活性。还观察到蛋白酶在不同表面活性剂和氧化剂存在下的高稳定性。此外，我们还研究了 EH2 从猪和鲑鱼皮胶原蛋白产生的水解产物的抗氧化活性。结果表明，鲑鱼皮胶原蛋白水解物比猪皮胶原蛋白具有更高的 DPPH（1,1-二苯基-2-三硝苯肼）（42.88% +/- 1.85）和羟自由基（61.83% +/- 3.05）清除活性。对于氧自由基吸收能力，猪皮胶原蛋白水解产物具有更高的效率（7.72+/-0.13μmolTE/μmol）。即使是1 nM的混合肽也能有效降低细胞内活性氧的水平。两种底物在水解3 h时发挥出最佳的抗氧化活性。水解时间和底物类型对水解产物的抗氧化性能有重要影响。蛋白酶水解肉胶原蛋白肽具有良好的抗氧化活性，这可能对海洋蛋白酶在生物催化行业的潜在应用具有启发意义。
The study reported on the isolation of a metalloprotease named EH2 from Pseudoalteromonas sp. H2. EH2 maintained more than 80% activity over a wide pH range of 5-10, and the stability was also nearly independent of pH. Over 65% activity was detected at a wide temperature range of 20-70 degrees C. The high stability of the protease in the presence of different surfactants and oxidizing agents was also observed. Moreover, we also investigated the antioxidant activities of the hydrolysates generated from porcine and salmon skin collagen by EH2. The results showed that salmon skin collagen hydrolysates demonstrated higher DPPH (1,1-diphenyl-2-picrylhydrazyl) (42.88% +/- 1.85) and hydroxyl radical (61.83% +/- 3.05) scavenging activity than porcine skin collagen. For oxygen radical absorbance capacity, the hydrolysates from porcine skin collagen had higher efficiency (7.72 +/- 0.13 mu molTE/mu mol). Even 1 nM mixed peptides could effectively reduce the levels of intracellular reactive oxygen species. The two types of substrates exerted the best antioxidant activity when hydrolyzed for 3 h. The hydrolysis time and type of substrate exerted important effects on the antioxidant properties of hydrolysates. The hydrolyzed peptides from meat collagens by proteases have good antioxidant activity, which may have implications for the potential application of marine proteases in the biocatalysis industry.