Pollen Viability and Longevity of Switchgrass (Panicum virgatum L.)
Pollen Viability and Longevity of Switchgrass (Panicum virgatum L.)
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DOI：
10.2135/cropsci2011.01.0057
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发表时间：
2011-11-01
期刊：
影响因子：
2.3
通讯作者：
Wang, Zeng-Yu
中科院分区：
文献类型：
--
作者：
Ge, Yaxin;Fu, Chunxiang;Wang, Zeng-Yu
花粉对于种子生产至关重要，并且是柳枝稷 (Panicum virgatum L.) 等异型杂交物种基因流动的主要手段。缺乏有关柳枝稷的基本花粉生物学的信息。本研究使用不同材料（包括四倍体品种 Alamo、八倍体品种 Cave-in-Rock 和转基因 Alamo 植物）研究了花粉活力、花粉寿命和花粉大小。花粉粒是从田间种植的阿拉莫植物和岩洞植物以及温室种植的转基因植物中收集的。花粉尺寸在42.5至54.0μm范围内；转基因植物和对照植物之间的平均花粉大小没有观察到显着差异。温度升高和紫外线 B 照射会对花粉活力和寿命产生负面影响，而相对湿度的影响有限。天气条件对花粉寿命有很大影响。在阳光充足的大气条件下，两个品种的花粉寿命均迅速下降，半衰期为
Pollen is essential for seed production and serves as the primary means of gene flow in outcrossing species like switchgrass (Panicum virgatum L.). There is a lack of information on basic pollen biology in switchgrass. This study investigated pollen viability, pollen longevity, and pollen size using different materials, including the tetraploid cultivar Alamo, the octoploid cultivar Cave-in-Rock, and transgenic Alamo plants. Pollen grains were collected from field-grown Alamo and Cave-in-Rock plants, and greenhouse-grown transgenics. Pollen size was in the range of 42.5 to 54.0 mu m; no significant difference was observed in average pollen size between transgenic and control plants. Increasing temperature and ultraviolet-B irradiation negatively affected pollen viability and longevity, while relative humidity had only limited impact. Weather conditions had a large impact on pollen longevity. Under sunny atmospheric conditions, pollen longevity of both cultivars decreased rapidly, with a half-life of