Cell divisions are not essential for the direct conversion of fibroblasts into neuronal cells
Cell divisions are not essential for the direct conversion of fibroblasts into neuronal cells
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DOI：
10.1080/15384101.2015.1012875
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发表时间：
2015-04-15
期刊：
影响因子：
4.3
通讯作者：
Serov, O. L.
中科院分区：
文献类型：
--
作者：
Fishman, V. S.;Shnayder, T. A.;Serov, O. L.
直接谱系转换是疾病建模和再生医学的一种有前途的方法。细胞分裂在体细胞重编程为多能性中起关键作用，然而它们在直接谱系转换中的作用尚不清楚。在这里，我们使用的成纤维细胞转分化成神经元细胞的强制表达的定义转录因子作为一个模型系统，研究细胞分裂的作用，在直接转换过程中。我们已经表明，转换发生在细胞周期抑制剂aphidicolin或mimosine的存在下。此外，细胞周期激活剂cMyc的过表达会对直接转化过程产生负面影响。总的来说，我们的研究结果表明，细胞分裂是不必要的成纤维细胞直接转化为神经元细胞。
Direct lineage conversion is a promising approach for disease modeling and regenerative medicine. Cell divisions play a key role in reprogramming of somatic cells to pluripotency, however their role in direct lineage conversion is not clear. Here we used transdifferentiation of fibroblasts into neuronal cells by forced expression of defined transcription factors as a model system to study the role of cellular division in the direct conversion process. We have shown that conversion occurs in the presence of the cell cycle inhibitors aphidicolin or mimosine. Moreover, overexpression of the cell cycle activator cMyc negatively influences the process of direct conversion. Overall, our results suggest that cell divisions are not essential for the direct conversion of fibroblasts into neuronal cells.