Erythrocyte aggregation under high pressure studied by laserphotometry and mathematical analysis
Erythrocyte aggregation under high pressure studied by laserphotometry and mathematical analysis
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通过激光光度测定和数学分析研究高压下红细胞聚集
DOI：
10.1016/j.colsurfb.2015.12.038
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Toshiaki Dobashi
中科院分区：
文献类型：
--
作者：
Yoshiharu Toyama;Hisashi Yoshida;Takao Yamamoto;Toshiaki Dobashi
本文用激光光度法和唯象分析法研究了静水压对红细胞聚集的影响。通过将猪红细胞悬浮在其自身血浆中制备样品。在实验装置中使用由不锈钢块组成的高压容器，不锈钢块具有用于容纳样品池的孔和两个蓝宝石窗口以允许He-Ne激光束通过。以1500 rpm搅拌悬浮液以均匀分散红细胞。在将搅拌速率从1500 rpm降低到300 rpm之后，立即在40-200 MPa的高压下每10 ms记录透射光强度（I）。由于红细胞聚集，I值随时间（t）增加。根据唯象理论，方程Δ I（t）= Δ Ieq [1-e-Kt/（1-B（1-e-K t））]是针对由于红细胞聚集引起的透射光强度变化（ΔI）推导的，其中Δ Ieq是稳态下的透射光强度，K是时间常数，B是常数，表示在时间t时红细胞聚集体上的相互作用位点的数目与稳态时红细胞聚集体上的相互作用位点的数目的比率。在所有压力下测得的ΔI随时间的变化与理论方程吻合得很好。Δ Ieq大致随压力的增加而增加。另一方面，K和B在120 MPa以上突然下降。当压力大于120 MPa时，团聚体的生长速率下降。这些结果表明，在约120 MPa的红细胞聚集的机制的变化。我们讨论了参数的物理意义。
The effects of hydrostatic pressure on erythrocyte aggregation have been studied by laser photometry and analysis based on a phenomenological theory. Samples were prepared by suspending swine erythrocytes in their own plasma. A high-pressure vessel consisting of a stainless-steel block with a hole to hold a sample cell and two sapphire windows to allows the passage of a He–Ne laser beam was used in the experimental setup. The suspension was stirred at 1500 rpm to disperse the erythrocytes homogeneously. Immediately after reducing the stirring rate from 1500 rpm to 300 rpm, the transmitted light intensity (I) was recorded every 10 ms under a high pressure of 40–200 MPa. The value of I increased with time (t) owing to erythrocyte aggregation. From the phenomenological theory, the equation Δ I (t)= Δ I eq [1− e− K t/(1− B (1− e− K t))] was derived for the change in the transmitted light intensity (ΔI) due to erythrocyte aggregation, where ΔI eq is the transmitted light intensity in the steady state, K is a time constant and B is a constant that represents the ratio of the number of interaction sites on erythrocyte aggregates at time t to that in the steady state. The observed time courses of ΔI obtained at all pressures could be closely fitted to the theoretical equation. ΔI eq roughly increased with increasing pressure. On the other hand, K and B abruptly decreased above 120 MPa. The growth rate of aggregates decreased above 120 MPa. These results suggest a change in the mechanism of erythrocyte aggregation at approximately 120 MPa. We discuss the physical meaning of the parameters.