Strain- and Substrate-Dependent Redox Mediator and Electricity Production by Pseudomonas aeruginosa
Strain- and Substrate-Dependent Redox Mediator and Electricity Production by Pseudomonas aeruginosa
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DOI：
10.1128/aem.01342-16
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发表时间：
2016-08-01
影响因子：
4.4
通讯作者：
Rosenbaum, Miriam A.
中科院分区：
文献类型：
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作者：
Bosire, Erick M.;Blank, Lars M.;Rosenbaum, Miriam A.
铜绿假单胞菌是微生物生物电化学系统（BES）中重要的、繁荣的微生物群落成员，它通过生产多功能的非那嗪氧化还原介质。模型菌株的纯培养实验揭示了铜绿假单胞菌与发酵微生物的协同作用，这种协同作用是通过共享发酵产物2,3-丁二醇介导的。我们在这里的工作表明，铜绿假单胞菌在介导电流生产中的行为和效率强烈依赖于铜绿假单胞菌的菌株。我们比较了先前研究的模型菌株P. aeruginosa PA14、替代模型菌株P. aeruginosa PAO1和BES分离的假单胞菌sp.菌株KRP1以葡萄糖和发酵产物2,3-丁二醇和乙醇为碳底物的产非那嗪水平。我们发现三种菌株在依赖底物的非那嗪产量和产生的阳极电流方面存在显著差异，其中BES分离物KRP1总体上是最好的电流产生者，并且在葡萄糖作为底物时表现出最高的电化学活性(19 μ a cm（-2)，类似于150 μ g ml（-1）的非那嗪羧酸作为氧化还原介质）。令人惊讶的是，P. aeruginosa PAO1在所有测试条件下都表现出非常低的非那嗪产量和电化学活性。微生物燃料电池和其他微生物电化学系统在废水处理和生物修复等环境技术方面具有很大的前景。虽然人们非常重视实现这种系统的材料和设备的开发，但对潜在微生物学和生态学的调查和更深入的了解却很落后。生理研究集中在纯培养系统中表现出直接电子转移的微生物。同时，由铜绿假单胞菌产生的天然氧化还原化合物介导的电子转移可能使整个微生物群落能够访问固体电极作为替代电子受体。为了更好地理解介质生产者和介质利用者之间的生态关系，我们在这里比较了三种假单胞菌菌株依赖非那嗪的电活性。这项工作为微生物燃料电池中介导电子转移的更复杂的共培养研究奠定了基础。
Pseudomonas aeruginosa is an important, thriving member of microbial communities of microbial bioelectrochemical systems (BES) through the production of versatile phenazine redox mediators. Pure culture experiments with a model strain revealed synergistic interactions of P. aeruginosa with fermenting microorganisms whereby the synergism was mediated through the shared fermentation product 2,3-butanediol. Our work here shows that the behavior and efficiency of P. aeruginosa in mediated current production is strongly dependent on the strain of P. aeruginosa. We compared levels of phenazine production by the previously investigated model strain P. aeruginosa PA14, the alternative model strain P. aeruginosa PAO1, and the BES isolate Pseudomonas sp. strain KRP1 with glucose and the fermentation products 2,3-butanediol and ethanol as carbon substrates. We found significant differences in substrate-dependent phenazine production and resulting anodic current generation for the three strains, with the BES isolate KRP1 being overall the best current producer and showing the highest electrochemical activity with glucose as a substrate (19 mu A cm(-2) with similar to 150 mu g ml(-1) phenazine carboxylic acid as a redox mediator). Surprisingly, P. aeruginosa PAO1 showed very low phenazine production and electrochemical activity under all tested conditions.IMPORTANCEMicrobial fuel cells and other microbial bioelectrochemical systems hold great promise for environmental technologies such as wastewater treatment and bioremediation. While there is much emphasis on the development of materials and devices to realize such systems, the investigation and a deeper understanding of the underlying microbiology and ecology are lagging behind. Physiological investigations focus on microorganisms exhibiting direct electron transfer in pure culture systems. Meanwhile, mediated electron transfer with natural redox compounds produced by, for example, Pseudomonas aeruginosa might enable an entire microbial community to access a solid electrode as an alternative electron acceptor. To better understand the ecological relationships between mediator producers and mediator utilizers, we here present a comparison of the phenazine-dependent electroactivities of three Pseudomonas strains. This work forms the foundation for more complex coculture investigations of mediated electron transfer in microbial fuel cells.