Inactivation of individual mammalian cells by single alpha-particles
Inactivation of individual mammalian cells by single alpha-particles
复制标题
DOI：
10.1080/095530097143176
复制
发表时间：
1997-10-01
影响因子：
2.6
通讯作者：
Gialanella, G
中科院分区：
文献类型：
--
作者：
Pugliese, M;Durante, M;Gialanella, G
目的：测量暴露于规定数量的 4.3 MeV α 粒子（轨迹平均 LET = 105 keV/μ m）后中国仓鼠 V79 细胞的克隆性死亡。材料和方法。在那不勒斯 TTT-3 Tandem 加速器上安装的放射生物学设施中，使用“Biostack”方法对细胞进行辐照，该方法可以仔细确定入射轨迹相对于细胞的位置。通过荧光显微镜鉴定亚细胞结构，通过 LR-115 固态核径迹检测器观察径迹。结果：细胞质中的粒子撞击不会显着影响细胞存活，但存活概率随着核遍历次数呈指数下降。测得一次 4.3 MeV α 粒子穿越细胞核后存活的概率为 0.67 +/- 0.10。失活横截面显着高于传统存活曲线的预期。然而，用泊松统计对数据进行折叠表明，如果一个α粒子穿过细胞核的平均值高于恰好一次粒子穿过后的测量值，则预期的存活水平较高。结论：V79细胞在细胞核中单次α粒子穿过后有大约67%的概率存活。一旦考虑到遍历的泊松分布，单粒子生存曲线与传统的剂量-生存关系一致。
Purpose: To measure clonogenic death of Chinese hamster V79 cells following exposure to a defined number of 4.3 MeV alpha-particles (track-averaged LET = 105 keV/mu m).Materials and methods. Cells were irradiated at the radiobiological facility installed at the TTT-3 Tandem accelerator in Naples by using a 'Biostack' approach, which allows the positions of incident tracks relative to cells to be carefully determined. Subcellular structure was identified by fluorescence microscopy, while tracks were visualized by LR-115 solid state nuclear track detectors.Results: Particle hits in the cytoplasm did not significantly affect cell survival, yet survival probability decreased exponentially as a function of the number of nuclear traversals. Measured probability of surviving to exactly one 4.3 MeV alpha-particle traversal in the cell nucleus was 0.67 +/- 0.10. Inactivation cross-section was substantially higher than expected from conventional survival curves. However, folding of the data with Poisson statistics showed that survival level expected if a mean of one a-particle goes through a nucleus is higher than the measured Value after exactly one particle traversal.Conclusions: V79 cells have about 67% probability to survive a single alpha-particle traversal in the cell nucleus. Single-particle survival curves are consistent with conventional dose-survival relationships, once Poisson distribution of traversals is taken into account.