Making Reporter Gene Constructs to Analyze Cis-regulatory Elements
Making Reporter Gene Constructs to Analyze Cis-regulatory Elements
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DOI：
10.1007/978-1-61779-228-1_23
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Luis Gomez-Skarmeta, Jose
中科院分区：
文献类型：
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作者：
Bessa, Jose;Luis Gomez-Skarmeta, Jose
顺式调控序列控制基因转录的时间、地点和数量。更好地了解这些元素是更好地了解发育、细胞分化和形态发生的基础。已经发展了几种基于电子分析或芯片序列实验的方法来检测顺式作用序列。在这里，我们描述了一种从基因组DNA中分离此类序列并将其克隆到表达载体中的协议，用于使用Gateway克隆技术进行功能分析。
Cis-regulatory sequences control when, where, and how much genes are transcribed. A better understanding on these elements is a fundamental keystone to better understand development, cell differentiation, and morphogenesis. Several methods based on in silico analysis or ChIP-seq experiments have been developed to detect cis-acting sequences. Here, we describe a protocol to isolate such sequences from genomic DNA and to clone them into expression vectors for functional assays using the Gateway cloning technology.