H pylori iceA alleles are disease-specific virulence factors
H pylori iceA alleles are disease-specific virulence factors
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DOI：
10.3748/wjg.v13.i18.2581
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发表时间：
2007-05-14
影响因子：
4.3
通讯作者：
Bagci, Huseyin
中科院分区：
文献类型：
--
作者：
Caner, Vildan;Yilmaz, Mustafa;Bagci, Huseyin
目的：为了表征和比较幽门螺杆菌菌株的基因型谱分离出的慢性胃炎和十二指肠溃疡患者在西部的Turkish.METHODS：共46例[30慢性胃炎（CG）和16十二指肠溃疡（DU）]谁接受了内镜检查，因为消化不良的投诉进行了研究。结果：43株（93.5%）幽门螺杆菌（Hp）cagA基因阳性。vac，41 s1m2基因型是CG和DU患者的主要亚型，分别占63.3%和68.7%。20株（66.6%）CG患者分离株为iceA2阳性，DU患者分离株以iceA1为主（68.8%）。cagA vacA s1m2基因型与iceA等位基因的相关性分析表明，cagA vacA s1m2 iceA 2基因型和cagA vacA s1m2 iceA 1基因型分别是CG和DU的流行基因型。结果发现cagA vacA s1m2基因型可能是CG和DU的共同毒力因子，而iceA等位基因则对胃十二指肠病变具有特异性。(C)2007年，WJG出版社。All rights reserved.
AIM: To characterize and compare genotype profiles of H pylori strains isolated from patients with chronic gastritis and duodenal ulcer in western part of Turkey.METHODS: A total of 46 patients [30 chronic gastritis (CG) and 16 duodenal ulcer (DU)] who had undergone endoscopy because of dyspeptic complaints were studied. The antral biopsy specimens were evaluated for the presence of H pylori by rapid urease test and culture, and the genotype profiles were determined by real-time PCR.RESULTS: The cagA gene was observed in 43 (93.5%) isolates. The vac,41 s1m2 genotype was the predominant subtype, found in 63.3% and 68.7% of isolates in patients with CG and DU, respectively. Twenty (66.6%) isolates from patients with CG were iceA2 positive while the iceA1 was predominant in those with DU (68.8%). In terms of the association of the iceA alleles to other genes, both alleles were significantly associated with the cagA vacA s1m2 genotype.CONCLUSION: The prevalent circulating genotypes in CG and DU were cagA vacA s1m2 iceA2 and cagA vacA s1m2 iceA1 genotype, respectively. It was found that cagA vacA s1m2 genotype seems to be common virulence factors in both CG and DU while iceA alleles show specificity for gastroduodenal pathologies in this study. (C) 2007 The WJG Press. All rights reserved.